摘要
目的:观察丹酚酸B对大鼠HgCl2中毒肾间质纤维化模型的作用,并探讨其影响肾脂质过氧化的部分作用机理。方法:雄性大鼠以8mg/kgHgCl2水溶液灌胃9周制备大鼠肾间质纤维化模型。从造模开始SA-B组以丹酚酸B10mg/kg灌胃,VitE组以100mg/kg灌胃,1次/天,共9周。试剂盒方法检测肾功能和脂质过氧化,肾组织HE染色、六胺银染色及Masson染色观察肾组织炎症与胶原纤维沉积变化;盐酸水解法测定肾组织羟脯氨酸(Hyp)含量。结果:丹酚酸B能明显减轻模型大鼠肾小管炎症坏死与肾间质胶原沉积,减轻肾组织Hyp含量,降低异常增高的血清尿素氮与肌酐水平,升高GSH含量,并降低MDA含量。结论:丹酚酸B可明显改善大鼠HgCl2中毒性肾间质纤维化,其机制为增强机体抗氧化损伤能力与减轻脂质过氧化损伤。
Objective：To investigate the effects and mechanisms of salvianolic acid- B （SA- B） against renal interstitial fibrosis in rats induced by administration of mercurto 4 groups： normal group, model group, SA - B treatment group and VitE treatment group. Renal, interstitial, fibrosis model was induced by administration of HgCl2 at a dose of 8mg/kg rat weight every morning for 9 weeks. Meanwhile rats in SA - B treatment group and ic chloride （ HgCl2 ）. Methods; The rats were randomly divided inVitE treatment group were treated with SA - B at a dose of 10mg/kg and VitE at a dose of 100mg/kg respectively in the afternoon for 9 weeks. Serum creatinine and urea nitrogen content and renal lipid oxidation injury, namely, activity of superoxide dismutase （SOD） and glutathione peroxidase （ GSH - Px） and content of GSH and malondiahdehyde （MDA） of kidney, were tested with commercial kits. Hydroxyproline （Hyp） content of kidney was assayed with Jamall＇s method; histological changes of kidney were observed with hematoxylin and eosin （H＆E） Stain, masson stain and methenamine silver （PASM） stain. Results： Compared to model group, SA -B improved renal function, decreased kidney Hyp content, alleviated renal interstitial fibrosis, increased GSH content and decreased MDA content in kidney. Conclusions： SA - B could ameliorate renal interstitial fibrosis in rats induced by HgCl2, which was related to increasing organism function against oxidative damage and alleviating lipid oxidation injury.
出处
《中药药理与临床》
CAS
CSCD
北大核心
2008年第1期12-15,共4页
Pharmacology and Clinics of Chinese Materia Medica
基金
上海市科委重点基础项目(04JC14609)
上海高校中医内科学E研究院建设项目(E03008)
教育部高等学校博士学科点专项科研基(20060268005)
上海市重点学科(Y0302)部分资助
关键词
丹酚酸B
氯化汞
肾间质纤维化
氧化损伤
Salvianolic acid. B, renal interstitial fibrosis
mercuric chloride
lipid oxidation
