摘要
通过研究不同磁处理对盐渍条件下海滨锦葵种子萌发的影响,初步探讨了磁处理提高盐渍条件下种子萌发率的机理。结果表明:盐胁迫对海滨锦葵种子萌发具有强烈的抑制作用,外加磁处理可以不同程度上恢复种子萌发率,其中以600mT处理30min效果最佳。300mmol/LNaCl处理明显降低了种子淀粉酶和异柠檬酸裂解酶(ICL)活性,呼吸速率也明显下降,外加磁处理后淀粉酶活性有一定程度恢复,但效果不显著;异柠檬酸裂解酶活性则明显恢复,通过对该酶动力学常数Km以及Vmax进行分析,发现磁处理后ICL与底物亲和能力及酶促反应的最大速度均得到显著提升;种子呼吸速率也显著上升。为进一步探讨磁处理提高盐胁迫下种子萌发率的原因,实验同时设计了与NaCl处理等渗透势的PEG处理试验,结果显示,磁处理只提高NaCl处理组种子萌发率。上述实验结果暗示,磁处理可能通过影响盐胁迫后植物细胞内离子转移和分布的变化,进而引起与之相关的代谢酶类、生理活性发生改变,从而提高了盐渍环境下海滨锦葵种子的萌发率。
Even though Kosteletzkya virginica is a halophytic species,it has a relatively low seed germination rate in heavy saline environment. Germination under salt stress in K. virginica and the physiological mechanism were studied. The results showed that salt stress had a strong inhibitory effect on seed germination; magnetic treatments with different intensity restored the germination rate in various degree,of which 600mT/30min achieved the best effect. 300mmol/L NaCl treatment significantly decreased seed-amylase,isocitrate lyase （ ICL） activities and respiration rate; magnetic treatment restored amylase activity to a certain extent,but strongly increased ICL activity; kinetic analysis on Km and Vmax of ICL showed that magnetic treatment improved not only the substrate affinity and the maximum speed of enzymatic reaction significantly,but also seed respiration rate. Iso-osmotic potential of PEG and NaCl experiment was designed to explore the mechanism further. The result showed that magnetic treatment only improved seed germination rate under NaCl stress. All above indicated that magnetic treatment might influence the transfer and distribution of ion in plant cells under salt stress,which further changed the associated metabolic enzymes and physiological activities,and increased seed germination rate of K. virginica in salt environment.
出处
《中国油料作物学报》
CAS
CSCD
北大核心
2010年第2期257-262,269,共7页
Chinese Journal of Oil Crop Sciences
基金
"十一五"国家科技支撑计划(2006BAD09A04
2006BAD09A08)
关键词
海滨锦葵
磁处理
盐胁迫
Kostelezkya virginica
Magnetic field treatment
Salt stress
