摘要
通过土壤培养试验、紫外吸收光谱和荧光光谱技术 ,研究了重金属汞对土壤酸性磷酸酶催化活性、动力学性质的影响及机理 .结果表明 ,汞对土壤酸性磷酸酶活性、酶促反应动力学性质均具有显著影响 .汞对土壤酸性磷酸酶的作用可能为非竞争与反竞争混合的抑制类型 .而汞对麦芽酸性磷酸酶的抑制作用为反竞争性抑制 .重金属汞通过改变土壤酸性磷酸酶分子色氨酸、酪氨酸残基的微环境致使酶活性中心的构象发生变化 。
By fluorescence and differential UV-absorption spectra, the effects of Hg^(2+) on the activity and kinetic property of soil acid phosphatase were studied in soil culture under laboratory conditions, the corresponding mechanism was discussed as well. The obtained results revealed that Hg^(2+) had significant influences on the above characteristics of soil acid phosphatase. Data for kinetic studies suggested that the interaction between Hg^(2+) and soil acid phosphatase probably be mixed types of non-and anti-competitive inhibition interactions, whereas Hg^(2+) was an anti-competitive inhibitor of acid phosphatase from wheat germ. In addition, Hg^(2+) changed the microenvironment of tryptophan and tyrosine groups of soil acid phosphatase, which were active sites of the enzyme and consequently inhibited the activity of the enzyme.
出处
《环境科学学报》
CAS
CSCD
北大核心
2004年第5期865-870,共6页
Acta Scientiae Circumstantiae
基金
国家重点研究发展规划 ( 973 )项目 ( 2 0 0 2CB4 1 0 80 0 )
