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免疫细胞化学在浆膜腔积液腺癌细胞与反应性间皮细胞鉴别诊断中的应用 被引量:2

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摘要 目的:探讨浆膜腔积液中转移性腺癌细胞与反应性增生间皮细胞的鉴别。方法:对70例浆膜腔积液采用常规细胞学涂片观察细胞学形态,并采用免疫细胞化学EliVision两步法检测E-cadherin、CEA、HBME-1、calretinin的表达。结果:转移性腺癌细胞44例,反应性增生间皮细胞26例。腺癌细胞与增生间皮细胞在形态上各具特点,但高分化腺癌细胞与增生间皮细胞仅从形态有时难以区分。免疫细胞化学显示E-cadherin、CEA对腺癌细胞的敏感性为80.0%、86.4%,特异性为92.3、100%;HBME-1、calretinin对增生间皮细胞的敏感性为84.6%、76.9%,特异性为97.7%、100%;两者对4种抗体的表达有显著差异。结论:常规涂片结合免疫细胞化学对鉴别腺癌细胞与增生间皮细胞有很大帮助,E-cadherin、CEA、HBME-1、calretinin是有效实用的抗体组合。
作者 陈金华
出处 《实用医学杂志》 CAS 北大核心 2010年第12期2100-2101,共2页 The Journal of Practical Medicine
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