摘要
目的探讨中药联合窄谱中波紫外线(NB-UVB)在调节寻常型银屑病皮损中角质形成细胞凋亡的可能作用。方法选择30例寻常型银屑病患者给予中药银屑病方联合NB-UVB治疗,疗程8周,取治疗前后标本,用免疫组化方法检测正常人(20名)及患者(30例)治疗前后皮损中B细胞淋巴瘤/白血病-2(Bcl-2)蛋白、天冬氨酸特异性半胱氨酸蛋白酶-3(Caspase-3)、生存素(survivin)的表达。结果寻常型银屑病患者皮损中Bcl-2(7.50±2.01)的表达明显低于正常组(12.65±2.83),Caspase-3(21.73±3.70)的表达明显高于正常组织(8.55±2.16),survivin(23.90±2.82)的表达明显高于正常组织(7.06±1.96,P<0.01);治疗后Bcl-2(13.63±2.14)表达上调,Caspase-3(11.70±2.44)、survivin(12.46±1.80)表达下调(P<0.01),三者均有向正常组织表达转化的趋势。银屑病面积和严重程度指数(PASI)评分由治疗前(14.24±3.42)下降至治疗后(3.52±1.07,P<0.01))。结论中药银屑病方联合NB-UVB可能通过调节Bcl-2、Caspase-3、survivin的表达而调节角质形成细胞的凋亡达到治疗目的。
Objective To reveal the possible role of combined therapy with Chinese drug and narrow broad ultraviolet B （NB-UVB） on keratinocytes apoptosis in skin lesion of psoriasis vulgaris （PV）. Methods Skin samples were taken from 20 healthy subjects and 30 PV patients before and after they received the combined therapy for 8 weeks. SP immunohistochemical method was used to detect the expressions of Bcl-2, Caspase-3 and survivin in the samples. Results As compared with those in the normal skin, expression of Bcl- 2 in PV skin was significantly lower （7.50 ±2.01 vs. 12.65 ±2.83）, while expression of Caspase-3 （21.73 ± 3.70 vs. 8.55 ±2.16）, and survivin （23.90 ±2.82 vs. 7.06 ±1.96） were higher （all P〈0.01 ）. After treatment, in skin of PV, Bcl-2 expression increased to 13.63 ±2. 14, Caspase-3 and survivin decreased to 11.70 ± 2.44 and 12.46 ± 1.80, respectively （all P 〈0.01 ）, showing a normalizing trend. Moreover, patients＇ psoriasis area and severity index （PASI） score decreased from 14.24 ±3.42 before treatment to 3.52 ± 1.07 after treatment （ P 〈0.01 ）. Conclusion The curing effect of the combined therapy with Chinese drug and NB-UVB in treating PV is possibly realized by way of regulating Bcl-2, Caspase-3 and survivin expressions to adjust keratinocyte apoptosis.
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2010年第7期706-709,共4页
Chinese Journal of Integrated Traditional and Western Medicine
