摘要
目的:应用免疫组化法,检测COX-2、TRX-1在乳腺癌组织中表达,探讨COX-2、TRX-1表达与乳腺癌临床病理特征及预后因素的关系。方法:122例乳腺癌组织石蜡标本制作成乳腺癌组织芯片。免疫组化SP法检测COX-2、TRX-1表达。所有患者均接受规范性手术、术后辅助化疗及放疗,激素受体阳性病人接受5年的内分泌治疗。从手术日期到复发转移或随访截止日期(2008年7月)确定为患者无病生存期。共有45例复发转移病例。结果:122例乳腺癌组织中COX-2、TRX-1均呈高表达,分别为58.2%、54.1%,且二者表达呈正相关(r=0.286,P=0.001);COX-2表达与临床分期(r=0.193,P=0.033)、肿块大小(r=0.192,P=0.034)及淋巴结转移(r=0.186,P=0.040)呈正相关,TRX-1表达与临床分期(r=0.206,P=0.023)、肿块大小(r=0.236,P=0.009)呈正相关,与淋巴结转移(r=0.175,P=0.054)不相关。COX-2或TRX-1表达与无病生存期呈负相关(P=0.027,P=0.046);COX-2和TRX-1共表达与无病生存期呈负相关(P=0.017)。结论:乳腺癌组织中COX-2、TRX-1均呈高表达,两者与乳腺癌患者预后密切相关;COX-2、TRX-1表达呈正相关,推测乳腺癌组织中TRX-1可能参与COX-2调节。
Objective ：To detect the expression of COX - 2 and TRX - 1 using SP methods to investigate the relationship between them,as well as with clinicopathological parameters,disease free survival time （DSF） in human breast cancer. Methods：Total of 122 breast cancer paraffin - embedded tissue was made into chip. These patients not only underwent completely resection but also accepted standard chemotheray or radiotherapy. Immunohistochemical staining SP method was performed. DFS ranged from the date of operation to relaps or follow - up （ July ,2008 ）. There were 45 patients relaped . Results：The expression rate of COX -2 and TRX - 1 was higher （58.2% ,54.1% ） and the correlation between them was positive（ r = 0. 286 ,P = 0.001 ）. Expression of COX - 2 was positive correlated with tumour size （ r = 0.192, P = 0. 034 ）, clinical stage （ r = 0. 193, P = 0.033 ）, lymph node metastasis（r =0. 186,P =0. 040）. Expression of TRX - 1 was positive correlated with tumour size（r =0.236,P =0. 009） and clinical stage （ r = 0.206, P = 0.023 ）, but not correlated with lymph node metastasis （ r = 0.175, P = 0.054）. Expression of COX - 2 or TRX - 1 or COX - 2 and TRX - 1 was negative correlated with DFS by Kaplan - Meier survival analysis （ P = 0. 027, P = 0.046, P = 0. 017 ）. Conclision： Over - expression of COX - 2 and TRX - 1 associated with a poor clinical outcome. In breast cancer TRX - 1 possibly regulates expression of COX -2.
出处
《现代肿瘤医学》
CAS
2010年第7期1300-1303,共4页
Journal of Modern Oncology
基金
辽宁省教育厅科技攻关课题(编号:2004D170)
辽宁省科技厅课题(编号:2004225004-12)
关键词
环氧化酶-2
硫氧还蛋白-1
乳腺癌
无病生存期
cyclooxygenase-2（COX -2）
thioredoxin-I（TRX-1）
breast cancer
disease free survival time（DFS）
