摘要
目的观察雌二醇(estradiol,E2)对体外培养骨髓来源内皮祖细胞(endothlial progenitor cells,EPCs)分化及凋亡的影响。方法密度梯度离心法获取骨髓单个核细胞,流式细胞分析、FITC-荆豆凝集素Ⅰ、DiI-乙酰化低密度脂蛋白荧光双染鉴定。单个核细胞培养7d后进行实验分为对照组、0.01μmol/LE2组、0.1μmol/LE2组、1μmol/LE2组。分别采用流式细胞仪、体外管型生成实验观察EPCs的凋亡及体外血管新生的变化。结果雌二醇治疗组体外血管新生实验评分分别为0.01μmol/L组(2.91±0.70);0.1μmol/L组(4.04±0.66);1μmol/L组(4.73±0.48),较对照组(2.13±0.59)均明显增加(P<0.01);流式细胞仪检测过氧化氢诱导的雌二醇治疗组EPCs凋亡率分别0.1μmol/L组[(0.24±0.01),(P<0.05)];1μmol/L组[(0.21±0.02),(P<0.01)],较对照组(0.29±0.02)明显降低;0.01μmol/L雌二醇治疗后EPCs凋亡率无明显变化[(0.27±0.03),(P>0.05)]。结论 E2可抑制EPCs凋亡、促进EPCs体外血管新生。
Objective To observe the effect of estradiol （E2） on apoptosis of endothelial progenitor cells （EPC） and in vitro neovascularization.Methods Bone marrow mononuclear cells （MNC）,harvested by density gradient centrifugation,were double-stained with FITC-agglutinin 1 and DiI-acetylated low density lipoprotein fluorescence and analyzed by flow cytometry.Seven days after culture,MNC were divided into control group,0.01 μmol/L E2 group,0.1 μmol/L group and 1 μmol/L E2 group.After treated with E2 at the concentrations of 0.01,0.1,and 1 μmol/L,apoptosis of EPC and in vitro neovascularization in experimental group were detected with a flow cytometer.Results E2 used at the concentrations of 0.01,0.01,and 1 μmol/L,promoted in vitro tube formation in experimental group and control group （2.91±0.70,4.04±0.66 and 4.73±0.48 vs 2.13±0.59）,and apoptosis of EPC in experimental group decreased （0.24±0.01 and 0.21±0.02 vs 0.29±0.02 and 0.29±0.02,P0.01）.Conclusion E2 can inhibit apoptosis of EPC and promote in vitro neovascularization.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2010年第13期1399-1402,共4页
Journal of Third Military Medical University
基金
国家自然科学基金(30470729,30800480)
留学回国人员启动基金(2009XHG13)
重庆市留学回国人员基金(2009BB5020)
第三军医大学创新青年基金(2007XG31)~~
关键词
雌二醇
内皮祖细胞
凋亡
血管新生
小鼠
estradiol
endothelial progenitor cell
apoptosis
neovascularization
