摘要
目的:研究共刺激分子B7H3分子在人髓性白血病细胞株U937和K562上的表达及其生物学意义。方法:应用流式细胞术及RT-PCR法检测B7H3分子在人髓性白血病细胞株U937和K562上的表达;并用鼠抗人B7H3单克隆抗体(mAb)作用于U937和K562细胞株,细胞计数法检测鼠抗人mAbB7H3对U937和K562细胞株生长的影响。结果:流式细胞术分析显示U937和K562细胞株均表达B7H3膜蛋白,RT-PCR检测到U937和K562细胞有B7H3mRNA产物;鼠抗人mAbB7H3对U937和K562细胞株有抑制生长的作用。结论:U937和K562细胞株组成性表达B7H3分子;鼠抗人mAbB7H3与U937和K562细胞表面的B7H3分子交联后可以抑制白血病细胞的生长。
Objective：To study the expression and function of B7H3 in human myeloid leukemic cell lines,U937 and K562. Methods：The protein and mRNA expression levels of B7H3 were detected by FACS and RT-PCR methods,respectively. The effect of B7H3 in human leukemic cell lines was studied by B7H3 mAb inhibiton assay. Cell proliferation was determined by cell counting. Results：B7H3 expressed in both U937 and K562 cell lines. B7H3 mAb could inhibit cell proliferation of those two cell lines. Conclusion：B7H3 is constitutive expression in human myeloid leukemia cell lines,U937 and K562. B7H3 mAb can conjugate with B7H3 expressed in cell surface,which plays a critical role in the survival and growth of leukemia cells.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2010年第8期1079-1081,1096,共4页
Journal of Nanjing Medical University(Natural Sciences)
