摘要
本研究旨在探讨蛋种鸡J亚群-禽白血病的发病机理。针对山东济宁某蛋种鸡场200日龄产蛋鸡的组织病料,分别进行组织病理学与超微病理学研究,检测禽白血病病毒(ALV-J)抗原与抗体,分离病毒,并对其进行单克隆抗体间接免疫荧光试验检测。患病鸡群出现渐进消瘦、瘫痪和最后死亡现象,全群死亡率达10%左右。病鸡的肝、脾等内脏器官明显肿大,表面可见大小不一的灰白色结节;其肾脏、脾脏、肺脏和肝脏等组织中有一定比例的胞质含红色嗜酸性颗粒的骨髓样细胞瘤细胞增生,也发现间质中淋巴细胞和成淋巴细胞浸润增生。同时在脾脏和法氏囊组织中均看到病毒颗粒,有囊膜,直径约100nm。病料中p27抗原和血清中抗体均呈阳性,并分离到ALV-J。以上结果提示该蛋种鸡群发生的肿瘤病是由ALV-J引起的。随着越来越多的禽白血病病例在蛋鸡中确诊,预示着我国蛋鸡业面临新的威胁,值得引起重视。
This experiment was conducted to study the mechanism of Subgroup J-avian leukosis of Egg-type Chicken Breeder. The disease samples from an egg-type chicken （200-day old） flock in Jining, Shandong Province were studied by histopathological and ultrastructural pathologic methods. The antigen and antibody of avian leukemia were tested by ELISA test kit. The virus was isolated and detected by Indirect immunofluorescence monoclonal antibody test. The sick flocks showed clinical signs of depression, loss of appetite, and extreme emaciation and they finally got paralysed and died. The mortality rate was about 10%. In the necropsy of five dead chickens, almost all livers and spleens showed moderate to severe enlargement with diffuse gray-white nodules; Histopathological examination showed that focal tumor cells, with spherical eosinophilic granules in the cytoplasm, were found in the kidney, spleen, lung, liver. Diffused lymphocytes and lymphoblasts were found in those tissues. At the same time, the virus particles were appeared in the spleen tissues and bursa of Fabricius, about 100 nm in diameter, which are enveloped and spherical. Results showed that p27 antigen and serum antibody were positive in the disease samples, and ALV-J was isolated from them. On this basis, we concluded that the disease in the egg-type chickens was caused by subgroup J Avian Leucosis virus. As there are more reports of ALV affection in commercial layer chickens, special attention should be called.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2010年第6期735-740,共6页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
科技部农业行业科技专项(200803019)
关键词
蛋种鸡
J亚群-禽白血病
组织病理学
超微结构变化
egg-type chicken breeder
subgroup J-avian leukosis
histopathology
ultrastructural lesions
