摘要
目的:研究IFN-γ+874位点T/A以及TNF-α-238位点G/A的单核苷酸多态性与HBV宫内感染的相关性。方法:采集HBV标记物单项或多项阳性孕妇的外周血,提取基因组DNA,根据新生儿是否感染HBV将孕妇分为宫内感染组和对照组。利用等位基因特异性PCR检测IFN-γ+874位点等位基因型,利用PCR-RFLP方法检测TNF-α-238位点等位基因型。结果:等位基因特异性PCR可以准确判断IFN-γ+874位点等位基因型,对照组IFN-γ+874位点等位基因频率A为0.562,T为0.438,而宫内感染组A为0.738,T为0.262,两组之间的差异具有统计学意义(χ2=4.38,P=0.036)。TNF-α-238位点等位基因型的检测可以使用PCR-RFLP方法,对照组TNF-α-238位点等位基因频率A为0.146,G为0.854,宫内感染组A为0.262,G为0.738,两组之间的差异无统计学意义(χ2=3.26,P=0.071)。结论:IFN-γ+874位点T/A等位基因与HBV宫内感染具有一定相关性,T等位基因对胎儿HBV宫内感染具有防护作用。TNF-α-238位点G/A等位基因型与HBV宫内感染的关系尚不明确。
Objective：To explore the correlation of single nucleotide polymorphisms of IFN-γ＋874 site and TNF-α-238 site with HBV intrauterine infection.Methods：To collect the peripheral blood from pregnant women with HBV infection,and they were divided into 2 groups of control group and intrauterine infection group based on whether the infants were infected by HBV.The allele of IFN-γ＋874 site was identified by allele-specific PCR,meanwhile,allele of TNF-α-238 site was identified by PCR-RFLP.Results：It was feasible to identify allele of IFN-γ＋874 site by allele-specific PCR,frequencies of allele of IFN-γ＋874 site were 0.562（A）and 0.438（T）in control group,there was significant difference（χ2=4.38,P=0.036）compared to intrauterine infection group with 0.738（A）and 0.262（T）.PCR-RFLP was an effective method to identify allele of TNF-α -238 site,no statistical difference（χ2=3.26,P=0.071）was observed in allele frequencies of TNF-α-238 site between control group with 0.146（A）and 0.854（G）and intrauterine infection group with 0.262（A）and 0.738（G）.Conclusion：There is correlation between allele T/A of IFN-γ＋874 site and HBV intrauterine infection,T allele can provide protective efficacy against HBV intrauterine infection,however,the correlation between allele G/A of TNF-α-238 site and HBV intrauterine infection is not obvious.
出处
《中西医结合肝病杂志》
CAS
2010年第3期131-134,共4页
Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases
基金
深圳市宝安科技计划项目(No.2008149)
关键词
干扰素-Γ
肿瘤坏死因子-α
单核苷酸多态性
肝炎病毒
乙型
宫内感染
interferon-gamma
tumor necrosis factor-alpha
single nucleotide polymorphism
hepatitis virus B
intrauterine infection
