摘要
目的 探讨HBsAg阳性孕妇及其新生儿外周血单个核细胞乙肝病毒 (PBMCHBV)复制状况。 方法 收集 2 0 0 1年 6月～ 2 0 0 2年 12月由太原市传染病院妇产科进行产前检查并分娩的 15 1例HBsAg阳性孕妇及其 15 3例新生儿 ,用选择性聚合酶链反应技术进行PBMCHBVrcDNA与PBMCHBVcccDNA的检测。结果 HBsAg阳性孕妇PBMCHBVrcDNA的检出率为 36 4 2 % (5 5 / 15 1) ,PBMCHBVcccDNA的检出率为 9 93% (15 / 15 1) ;新生儿PBMCHBVrcDNA的检出率为 2 2 88% (35 / 15 3) ,PBMCHBVcccDNA的检出率为 4 5 8% (7/ 15 3)。结论 PBMC为HBV的肝外复制场所 。
Objective In order to explore the replication status of PBMC HBV of HBsAg positive pregnant women and their newborns.Methods 151 pregnant women and 153 newborns were collected between 2001 June and 2002 December in Infectious Hospital of Taiyuan city.PBMC HBV DNA were determined by selected polymerase chain reaction (sPCR).Results 15(9.93%) out of 151 HBsAg positive pregnant women had HBV cccDNA in their PBMC,7(4.58%) out of 153 newborns had HBV cccDNA in their PBMC,55(36.42%) out of 151 HBsAg positive pregnant women had HBV rcDNA in their PBMC,35(22.88%) out of 153 newborns had HBV rcDNA in their PBMC.Conclusion PBMC was the replication site of HBV out of liver.PBMC HBV replicatlon of newborns was related to the replication of their mothers.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2004年第5期513-514,共2页
Chinese Journal of Public Health
基金
20 0 0年国家自然科学基金资助 (30 0 70 699)
关键词
乙型肝炎病毒
选择性聚合酶链反应
孕妇
HBSAG
hepatitis B virus
replication
selected polymerase chain reaction
prenant woman
HBsAg
