摘要
用稳态荧光探针标记技术动态观察内毒素休克大鼠肝亚线粒体质子跨膜转运的变化．发现，休克５ｈＡＴＰ、ＮＡＤＨ和琥珀酸钠所致的９氨基６氯２甲氧基吖啶（ＡＣＭＡ）最大荧光淬灭值（ΔＡｍａｘ）显著低于对照组（Ｐ＜００５）、最大荧光淬灭时间（ＴΔＡｍａｘ）、半数荧光淬灭时间（Ｔ１／２ΔＡｍａｘ）非常显著延长（Ｐ＜００１），肝线粒体质子跨膜转运能力下降；膜脂分子烃链和膜脂深层次流动性下降；线粒体膜ＰＬＡ２活性增加；血浆脂质过氧化产物ＭＤＡ和线粒体ＭＤＡ含量均显著增加．可能膜脂质过氧化和磷脂酶Ａ２的水解是引起内毒素休克肝线粒体质子转运功能改变的重要因素．
The changes of proton translocation across the inner mitochondrial membrane of
hepatocytes in endotoxic shock rats were studied.It was showed that: Five hours after shock,
the maximum value of fluorescent quenching( ΔA max )of ACMA(9 anmino 6 chloro 2
meto acridine) induced by ATP,NADH and succinate was significantly decreased ( P <0 05).
The time of maximum fluorescent quenching ( T ΔA max )and the time of half
maximum fluorescent quenching( T 1/2ΔA max )were significantly prolonged ( P <0
01). It was suggested that the ability of proton translocation across the IMM in endotoxic
shock rats was decreased . The membrane fluidity were significantly decreased at the deep
level and hydrocarbon chain. The mitochondrial membrane associated PLA 2 activity,
plasma MDA,and mitochondrial MDA contents were significantly increased in endotoxic
shock rats. The results showed that PLA 2, and lipid peroxide may be the causes of the
changes of proton translocation in endotoxic shock rats.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
1999年第1期43-47,共5页
Progress In Biochemistry and Biophysics
基金
军队"九五"攻关重点课题
关键词
线粒体
质子
内毒素休克
mitochondria, proton,
endotoxic shock
