摘要
以位于湖南省新化和宁乡县两个稻田肥力长期定位试验点的土样为材料,研究了不同施肥处理对稻田土壤有机氮组分、微生物生物量及功能多样性的影响.结果表明:与不施肥处理(CK)相比,化肥配施有机肥处理提高了稻田土壤酸解总氮(TAHN)及其组分中氨基糖氮(ASN)、氨基酸氮(AAN)和酸解氨态氮(AN)的含量,不同施肥处理对组分中酸解未知氮(HUN)的影响不尽相同.与CK相比,单施化肥处理对土壤微生物生物量碳、氮(MBC、MBN)含量的影响较小,化肥配施有机肥处理则显著提高了土壤MBC和MBN的含量.采用BIOLOG法对土壤微生物群落功能多样性进行测定,结果表明:中、高量有机肥处理提高了稻田土壤微生物的碳源利用率和微生物群落功能多样性;土壤微生物碳源利用的类型因长期不同施肥处理而产生差异.
Soil samples were collected from the plow layers at two long-term experiment sites in Xinhua and Ningxiang counties of Hunan Province,China to study the effects of long-term fertilization on organic nitrogen,microbial biomass,and microbial functional diversity of paddy soils. Long-term fertilization showed great effects on the soil N content. Compared with CK,treatments NPK plus manure or straw increased the contents of soil total acid-hydrolysable N and its fractions amino sugar N,amino acid N,and ammonium N. Treatment NPK had no significant effects on soil microbial biomass C and N,but treatments NPK plus manure increased the contents of soil microbial biomass C and N significantly. BIOLOG test showed that treatments NPK plus manure enhanced the carbon utilization efficiency of soil microbes,and improved the functional diversity of soil microbial communities,compared with CK. Long-term different fertilizer treatments resulted in the differences of carbon substrate utilization patterns of soil microbial communities.
出处
《应用生态学报》
CAS
CSCD
北大核心
2010年第6期1477-1484,共8页
Chinese Journal of Applied Ecology
基金
国家科技支撑计划项目(2008BADA7B01)
中国科学院知识创新工程项目(KZCX2-YW-423)资助
关键词
长期施肥
稻田土壤
有机氮
微生物生物量
功能多样性
long-term fertilization
paddy soil
organic nitrogen
microbial biomass
functional diversity.
