摘要
克雷伯氏菌(Klebsiella pneumoniae)是1,3-丙二醇(1,3-PD)生产菌。研究了经60Co诱变获得的一株产物耐受突变株KpA6010进行了发酵初期供氧时间、供氧与否和发酵过程中后期维持甘油浓度对1,3-PD生产及副产物形成影响。结果显示,采取全厌氧发酵和中后期维持甘油质量浓度20 g/L的工艺对1,3-PD生产有利。5 L罐全程厌氧补料分批培养结果表明,1,3-PD的产量、摩尔得率和生产强度分别达到70.81 g/L、0.649和2.083 g/(L.h),较出发菌分别提高了54.3%、52.0%和50.7%。
A mutant strain of Klebsiella pneumoniae with improved tolerance to 1,3-propanediol（1,3-PD） and acetate was isolated from medium containing selective pressure after（）^（60）Co irradiation.In this study,several fermentation processes,including different aeration time,anaerobic process,and glycerol concentration to be controlled during the mid-and-late fermentation phase,were conducted to investigate their effects on microbial metabolism.Results showed that the process without aeration and maintaining about 20 g/L glycerol concentration during the mid-and-late fermentation phase was suitable for 1,3-PD production.The fed-batch fermentation in a 5 L bioreactor showed that the production of 1,3-PD reached 70.81 g/L,the mol yield of 1,3-PD on glycerol was 0.649 and the productivity was 2.083 g/（L·h）,which were increased by 54.3%,52.0% and 50.7% respectively compared with the results of parent strain.
出处
《华东理工大学学报（自然科学版）》
CAS
CSCD
北大核心
2010年第3期367-371,共5页
Journal of East China University of Science and Technology
基金
江苏省高技术计划项目资助(BG2006036)
