摘要
克雷伯杆菌在含有盐酸氨基脲和甘油的磷酸盐缓冲液培养基中有氧发酵,可实现3-羟基丙醛(3-HPA)的转化。考察了影响3-HPA积累和稳定性的一些主要因素,如菌种培养时间、接种量、甘油浓度、盐酸氨基脲、培养温度和pH值。优化后的培养条件为:菌种培养时间24 h、接种量2.16 g／L、甘油30 g／L、盐酸氨基脲26.8 g／L、28℃和PH值7.2。在优化条件下培养27 h,发酵液中3-HPA质量浓度达到15.48 g／L,总得率和转化率分别为0.60 g／g和75％。3-HPA低温条件下比较稳定,pH值对其稳定性影响不大。
When Klebsiella Pneumoniae DSM 2026 were grown aerobically on a rich glycerol medium and then suspended in buffer supplemented and glycerol, aerobic conversion of glycerol to 3-hydroxypropionaldehyde (3-HPA) could be carried out. Several factors that may influence 3-HPA accumulation and the stability of 3-HPA were studied. The 3-HPA accumulation is greatly affected by cell age, biomass, glycerol concentration, semicarbazide hydrochloride concentration, temperature as well as pH value. The optimal conditions are obtained as follows: 12 h of cell age, 2.16 g/L of inoculum, 30 g/L of glycerol, 26.8 g/L of semicarbazide hydrochloride, at 28°C and 7.2 of pH value. When the strain grew in the medium under the optimal conditions for 27 h, 15.48 g/L of 3-HPA could be produced at an overall yield of 0.60 g/g, representing a 75% conversion rate. In general, 3-HPA is stable under cool temperature with a large range of 6 - 11 of pH value.
出处
《现代化工》
EI
CAS
CSCD
北大核心
2004年第11期36-39,共4页
Modern Chemical Industry
基金
国家自然科学基金(20276026)福建省自然科学重点项目(D0120002)基金资助
关键词
3-羟基丙醛
甘油
发酵
<Keyword>3-hydroxypropionaldehyde
glycerol
fermentation
