摘要
采用RT-PCR和RACE技术,从毛竹中克隆了C4H基因cDNA全长序列。该基因包含1 506 bp开放读码框,编码502个氨基酸。与NCB I核酸和蛋白数据库中序列进行比对,结果表明该基因与单子叶植物高粱、水稻和玉米中的C4H基因同源性较高,分别达到88%、88%和87%。经过荧光定量PCR分析,该基因在毛竹不同发育时期和不同组织中表达丰度不同,在冬笋中表达量最高,其次为春笋顶部、中部、叶、3年生茎、根、叶鞘、2年生茎、春笋、1年生茎,而在春笋基部中表达最低。在笋顶部、中部的高水平表达表明本文克隆的C4H基因与发育时期维管组织的细胞壁加厚相关,可以作为调控木质素合成的目标基因用于竹子基因工程改良。
The full-length cDNA encoding C4H gene was cloned from 1-year-old moso bamboo seedings cDNAs using RT-PCR and RACE methods.The whole open reading frame C4H gene was 1 506 bp encoding 502 amino acids.The amino acid sequence shared high similarity with the corresponding genes in monocots Sorghum vulgare,Oryza sativa and Zea mays at 88%,88% and 87% level,respectively.Real time quantitative PCR showed that expression of C4H was the highest in winter-shoot,moderate in top-spring shoot,mid-spring shoot,leaf,3-year-old stem,root,leaf sheath,2-year-old stem,whole spring shoot and 1-year-old stem,the lowest in base-shoot.This indicated that the cloned C4H was involved in the cell wall deposition during the development of vascular tissues,thus could be used in modulation of bamboo lignin biosynthesis through genetic engineering.
出处
《林业科学研究》
CSCD
北大核心
2010年第3期319-325,共7页
Forest Research
基金
十一五国家科技支撑项目2006BAD19B0203
863项目2006AA100109
关键词
毛竹
木质素
C4H
荧光定量PCR
Phyllostachys edulis
C4H
lignin
Real-time quantitative PCR
