摘要
目的：以酶联免疫细胞化学技术显示培养的雪旺氏细胞。方法：采用抗Ｓ－１００蛋白的小鼠单克隆抗体，以酶联抗小鼠抗体，联苯胺、过氧化氢显色。结果：雪旺氏细胞整个胞体及突起呈深棕褐色，成纤维细胞未见着色呈透明状态。结论：Ｓ－１００蛋白的单克隆抗体酶联免疫细胞化学技术，可特异性地显示培养的雪旺氏细胞。
Objective:To show the cultured Schwann's cell,it was stained by immunocytochemistry with anti-S-100 protein. Method:The Schwann's cell was showed by indirectly immunocytochemistry with mouse anti-S-100 protein McAb.Result:The Schwann's cells were stained brown, but fibroblasts stainless.Conclusion:The cultured Shwann's cell can be showed particularly by indirectly immunocytochemistry with anti-S-100 protein.
出处
《南通医学院学报》
1999年第1期10-11,共2页
ACTA Academiae Medicinae Nantong
基金
国家杰出青年科学基金
江苏省青蓝工程资助
