摘要
目的获得组织工程化的乳鼠许旺细胞.方法采用出生1 d乳鼠的脊髓后根冲经节,以复合酶消化法进行乳鼠许旺细胞培养.通过Sigma-cote处理滴管和加入神经生长因子减少细胞损失、加速细胞分裂、增加细胞总量;通过低浓度胰酶快速消化、差速贴壁法的有机结合对许旺细胞进行纯化;继用抗S-100蛋白单抗通过(即用型)ABC免疫组化检测试剂盒,SABC-CY3试剂盒进行细胞鉴定.结果细胞纯度＞90%,细胞量＞2×10 7/ml.结论本实验方法具有高度重复性,可获得高纯度,大量的许旺细胞.
Purpose To develop a method to culture Swchwann cells for tissue engineering.Methods One-day-old rats were sacrificed by decapitation and their Dorsal Root Ganglions were removed for primary culture.By adding nerve growth factor in dish and Sigma-cote painting burettes,quality of Schwann cells can be improved.By rapid low concentration trypsinization and differential adhesion,purity of ceils can also be improved.The cells were identified with S-100 protein antibody by ABC methods and SABC-CY3 methods.Results The purity of ceils was more than 90% and the quality was more than 2×10~7/ml.Conclusion These methods to cultivate Swchwann cells for tissue engineering were repeatable,high purity and quality cells can be harvestd.
出处
《中国眼耳鼻喉科杂志》
2005年第4期220-221,i003,共3页
Chinese Journal of Ophthalmology and Otorhinolaryngology
基金
上海市科委基金资助(020J14026)
