摘要
研究了小菜蛾颗粒体病毒(Plutella xylostella granulovirus,PxGV)对小菜蛾幼虫体内的酚氧化酶(PO)和保护酶活力的影响.结果表明,颗粒体病毒感染后,虫体内PO活力与对照组相比相对升高.对照组虫体内PO活力一直随日龄增大而下降;感染组虫体内前3 d PO活力也随日龄增大而下降,第5天降至最低点,第6天后又反弹升高.PxGV感染对虫体3种保护酶的影响程度各不相同.其中,过氧化氢酶(CAT)活力在对照组和感染组虫体中均随日龄增大而升高;同时,感染组虫体CAT活力显著高于对照组.相比之下,PxGV感染对小菜蛾幼虫体内过氧化物歧化酶(SOD)和过氧化物酶(POD)活力变化的影响较小.
2^nd-instar larvae of diamondback moth, Plutella xylostella （L.） were feed with occlusion bodies of Plutella xylostella granulovirus （PxGV） to study effects of the virus on the activities of phenoloxidase （PO） and three protective enzymes in the insect. It was shown that, PO activity in the larvae dropped down in the first three days post infection, and further down to the bottom level in the fifth day following a significant rising in the fourth day. After that, the enzymatic activity rose up in the sixth day and stabilized until seventh day when the larvae started dying. In the control, PO activity in the larvae, which were not attacked by the virus, kept dropping down up to the end. PO activity in the larvae infected by PxGV was always much higher than that in the larvae uninfected. Among the three protective enzymes, namely catalase （CAT）, superoxide dismutase （SOD）, and peroxidase （POD）, CAT activity in the larvae infected by PxGV kept rising from the first day to seventh day, and was significantly higher than that in the larvae uninfected. SOD and POD were less affected by infection.
出处
《华中师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2010年第2期274-277,283,共5页
Journal of Central China Normal University：Natural Sciences
基金
国家自然科学基金资助项目(30870115)
关键词
小菜蛾
小菜蛾颗粒体病毒
酚氧化酶
保护酶
Plutella xylostella L.
Plutella xylostella granulvirus
phenoloxidase
protective enzymes
