摘要
在溶液培养条件下,利用15N示踪法研究了不同形态氮对水稻植株氮损失的影响,并分析了影响氮损失的因素。水稻幼苗先在以15N为氮源的营养液中生长2周,然后转入供给不同氮形态的营养液中培养10 d。结果表明:供给NH4+-N的水稻长势最好,收获时地上部和根部生物量均高于其他氮形态处理,但其氮损失量也最大,损失率达到17.06%;供给复合氮源NH4NO3的水稻生物量和供给NH4+-N的相差不大,然而其氮损失率却显著下降,仅为9.96%,说明供给复合氮源可在不影响水稻生长的条件下,降低植株氮损失,提高其氮肥利用率。此外,供给NH4+-N的水稻叶片中NH4+含量、谷草转氨酶活性及叶片组织pH值均高于其他氮形态处理,表明植物体内NH4+浓度增加而引起的氨挥发可能是导致植物氮损失的原因之一。
15N tracer method was adopted to study the effects of different nitrogen forms（NH4^＋-N,NO3——N and NH4NO3） on the N loss of rice plant under the solution culture,and the factors affecting the N loss were analyzed.Firstly,rice seedlings were cultured in nutrient solution and fed with 15N（abundance 10%） for two weeks,and then transferred to the nutrient solution culture with different nitrogen forms for 10 d.The results showed that the rice with application NH4^＋-N had the highest biomass and the largest amount of N loss with the N loss rate of 17.06% compared with other nitrogen forms.The rice supplied the complex nitrogen source（NH4NO3） had no significant differences in biomass compared with that of NH4^＋-N,but the N loss rate decreased significantly with only 9.96%.The results indicated that the application of NH4NO3 decreased N loss of rice plant,and improved the nitrogen utilization efficiency without affecting the rice growth.In addition,NH4^＋ content,GOT activity and pH values of rice leaves applied with NH4^＋-N were higher than other nitrogen forms,indicating that ammonia volatilization resulted from the increase of NH4^＋ content of plant may be the main cause of the N loss of the plant.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
2010年第3期312-316,共5页
Journal of Huazhong Agricultural University
基金
国家科技支撑计划项目(2008BADA4B08-02)资助
关键词
水稻
氮形态
植株氮损失
Oryza sativa
nitrogen forms
N loss of the plant
