摘要
用CO2激光对小麦种子分别辐照0、1、3、5min,待其生长至12d时,用10%(W/V)PEG6000胁迫其幼苗,研究激光预处理对PEG6000水分胁迫下小麦幼苗根部脂质过氧化伤害的防护作用。结果表明,CO2激光预处理3min可使水分胁迫的小麦幼苗根部MDA、H2O2含量和O2.-产生速率显著降低(P<0.05),可显著提高(P<0.05)小麦幼苗根部SOD、POD、CAT、APX活性和根长、根干重。激光预处理3min可抑制由水分胁迫引起的小麦幼苗根部脂质过氧化作用。
The objective of this experiment was to study changes of antioxidative enzymes and lipid peroxidation in root of wheat seedlings exposed to water stress,as affected by CO2 laser （wavelength 10600 nm,power density 20.1 mW/mm2） pretreatment.Wheat seed embryos were exposed to CO2 laser radiation for 0,1,3 min and 5 min,respectively,and when the seedlings were 12 days old they were treated with 10% （W/V） PEG6000 solution for 8 days.Changes in the concentration of malondialdehyde （MDA） and hydrogen peroxide （H2O2）,the production rate of superoxide radical （O- .2） and superoxide dismutase （SOD）,peroxidase （POD）,catalase （CAT）,ascorbate peroxidase （APX） activities of root and the growth parameters of root （root length and root dry weight） were measured to test the effects of laser pretreatment.The results showed that suitable laser pretreatment of embryos had positive effect on the root of wheat seedlings subjected to water stress,such as decreasing the concentration of MDA and H2O2,the production rate of O- . 2 and increasing the activities of SOD,POD,CAT and APX of root and root length and root dry weight.It was suggested that those changes in MDA,H2O2 and O2,anti-oxidative enzymes of root and root growth and development were responsible for protection wheat against water induced oxidative damage.
出处
《武汉植物学研究》
CSCD
北大核心
2010年第1期63-66,共4页
Journal of Wuhan Botanical Research
基金
国家自然科学基金资助项目(30670269)
河南省生物化学与分子生物学重点学科资助项目
河南师范大学青年科学基金资助项目(2008qk15)
关键词
CO2激光
水分胁迫
小麦
根
抗氧化酶
CO2 laser
Water stress
Wheat
Root
Antioxidative enzymes
