摘要
将罗氏沼虾随机分成5组,每组3个平行,每个平行1000尾,第1组为对照组,投喂基础日粮;另外4组为试验组,在基础日粮中分别添加0.05%、0.1%、0.2%、0.4%大黄蒽醌提取物。饲养10周后,对罗氏沼虾进行连续35℃高温应激48h,测定肝胰腺总抗氧化能力、过氧化氢酶、超氧化物歧化酶、丙二醛、一氧化氮以及应激蛋白70的mRNA相对含量变化。结果表明:应激前,0.05%组显著提高了肝胰腺过氧化氢酶活性、超氧化物歧化酶活性,显著降低了肝胰腺丙二醛含量;0.2%试验组显著增加了肝胰腺总抗氧化能力、一氧化氮浓度,显著降低了肝胰腺丙二醛含量;0.10%、0.2%试验组显著增加HSP70mRNA的相对含量;高温应激后,各组肝胰腺总抗氧化能力、过氧化氢酶活性、超氧化物歧化酶活性、一氧化氮浓度呈现降低趋势,其中0.1%和0.2%大黄蒽醌提取物相对较高,对照组较低;肝胰腺丙二醛含量呈现增加趋势,其中加0.1%和0.2%大黄蒽醌提取物比对照组低。应激6h后0.1%和0.2%大黄蒽醌提取物组的HSP70mRNA的相对含量仍比对照组高。因此添加0.1%和0.2%大黄蒽醌提取物提高了虾的抗氧能力,促进HSP70mRNA的相对含量,对高温应激有一定的保护作用。
Macrobrachium rosenbergii, also known as the giant river prawn,the giant freshwater prawn or the Malaysian prawn,is the most important cultivated species of freshwater shrimp in the world. This species （as well as other Macrobrachium ）is commercially important for its value as a food source. In China, the production of M. rosenbergii has been increasing in recent year. However, cultured M. rosenbergii in China as well as in other parts of the world have suffered from various stress factors such as ambient temperature, stocking density, physicochemical parameters, transport, and storage. Stress means the sum of nonspecific responses of organisms to various acute stimuli inside and outside. It is a physiological response of any organisms in the long evolution process to extend its adaptation, but excess response, which inhibits immunity, improves the free radical contents, destroys the homeostasis between producing and cleaning-up of free radical, and attacks the double bond of polyunsaturated fatty acid in plasma membrane, the last leading to increase the lipid peroxidation contents and lipid peroxidation injury. So these are many disadvantageous impacts on organisms and it causes fish to infect diseases even to die. Therefore, how to alleviate stress response is the research focus in this field. The present research falls on substances, which can strengthen immunity of organisms and alleviate stress, e. g. vitamin C, vitamin E,lipid acid, metal ion, fructose, astacin. Chinese herbal medicines have many merits：natural, highly effective, low toxicity, low side effects, and rich in resources. Chinese herbal medicines have many effective components, such as organic acids, alkaloid, saccharide ,volatile oil,wax, glycoside, tannic acid substance and some unknown factors that can elevate fish immunity and enhance the growth. However, few reports have been found in studying the effects of Chinese herbal medicines on the hepatopancreas antioxidative capacity and the level of HSPTO mRNA under high temperature of M. r
出处
《水产学报》
CAS
CSCD
北大核心
2010年第6期792-800,共9页
Journal of Fisheries of China
基金
农业科技成果转化资金项目(2008GB23260402)
International Foundation for Science
IFS(A/4396)
国家"十五"攻关项目(2004BA526B0505)
中央级公益性科研院所基本科研专项资金项目(2007JBFB11)
关键词
罗氏沼虾
大黄蒽醌提取物
抗应激
抗氧化
热应激蛋白70
Macrobrachium rosenbergii
anthraquinone extract from Rheum officinale Baill
stress prevention
antioxidative capacity
heat stress protein 70
