摘要
目的利用GC/MS、GC/NPD与固相萃取(SPE)技术相结合,建立血液中氯胺酮的定性定量分析方法。方法选择4-苯基丁胺为内标,采用Bond-Elut Certify固相柱萃取、二氯甲烷:异丙醇:氨水(78∶20∶2,v/v/v)洗脱的固相萃取分离技术,比较不同pH体系、洗脱溶剂对回收率的影响,建立血液中氯胺酮的GC/MS、GC/NPD定性定量分析方法。结果以GC/NPD分析氯胺酮在6.0～5000ng/mL范围内线性关系良好,GC/MS-Scan定性检测限为20.0ng/mL。方法平均回收率达96.9%,标准偏差小于5%。结论此方法可满足氯胺酮毒品滥用者血液定性定量分析。
Objective To develop a qualitative and quantitative analysis method for the determination of ketamine in blood samples using solid-phase extraction combined with GC/MS and GC/NPD. Methods Using 4-PBA as the internal standard,and the samples were extracted by solid-phase extraction with Bond-Elut Certify cartridge. Analytes were eluted by dichloromethane ：isopropanol ：ammonium hydroxide （78：20：2,v/v/v）. The optimum conditions for extraction,such as pH and eluent were studied in detail. Abuse drugs extracted from blood by solid phase extraction were detected and quantified by GC/MS and GC/NPD. Results The experimental results indicated that linear calibration curve were obtained within the range of 6.0～5000ng/mL,by GC/NPD and showed lower detection limit at 20.0ng/mL by GC/MS for ketamine in blood. The method recovery were 96.9%,and the standard deviation was less than 5%. Conclution The method may be applied for qualitative and quantitative analysis of ketamine in the drug abuse blood.
出处
《中国法医学杂志》
CSCD
北大核心
2010年第3期169-171,共3页
Chinese Journal of Forensic Medicine
基金
十一.五国家科技支撑计划项目(2006BAK-09B06)
