摘要
分别采用碱性硫酸铜和乙酸锌-亚铁氰化钾作为沉淀剂对雪糕样品进行预处理,并按照GB/T5009.35-2003第一法中的聚酰胺吸附法对样液中的色素进行提取,用高效液相色谱法进行合成色素含量的测定。结果表明,采用沉淀剂对样品进行预处理,可降低雪糕浆料的粘稠度,有利于色素的提取。对于四种常用合成色素柠檬黄、胭脂红、日落黄、亮蓝,以碱性硫酸铜作为沉淀剂,加标回收率为72.04%-82.64%,相对标准偏差为1.48%-3.50%;以乙酸锌-亚铁氰化钾作为沉淀剂,加标回收率为81.62%-95.73%,相对标准偏差为1.46%-3.19%。采用乙酸锌-亚铁氰化钾进行样品预处理的方法准确度和精密度良好,可作为高效液相色谱法测定雪糕中合成色素的样品预处理方法。
In order to detect the synthetic colorants content in ice cream bar with HPLC,samples were pretreated with precipitator alkali cupric sulfate or zincic acetate-potassium ferrocyanide firstly.Then synthetic colorants were separated according to the method of polyamide absorption,which is illustrated in the first method of GB/T5009.35-2003.Results showed that the ropiness degree of a sample was reduced by precipitation pretreatment,which results in effective separation of the colorants.The recovery rates of four commonly used synthetic colorants,tatrazine,ponceau,sunser yellow and brilliant blue were between72.04%-82.64% andRSD between1.48%-3.50% when alkali cupric sulfate was used.As to zincic acetate-potassium ferrocyanide,the values were between 81.62%-95.73% and 1.46%-3.19% correspondingly,which means better accuracy and precision.So the latter precipitator can be recommended for sample pretreatment in determination of synthetic colorants in ice cream bar with HPLC.
出处
《中国食品添加剂》
CAS
北大核心
2010年第3期228-231,共4页
China Food Additives
基金
广东药学院科研启动基金项目(2007GGW05)
关键词
雪糕
合成色素
高效液相色谱
预处理
沉淀剂
ice cream bar
synthetic colorant
HPLC
pretreatment
precipitator
