摘要
目的研究苯及其代谢产物氢醌对体外培养人淋巴细胞的遗传毒性。方法用常规离心方法分离淋巴细胞,离体培养24h后加S9液,设置苯低、中、高浓度(20、300、5000μl/L)和氢醌低、中、高浓度(50、150、450μmol/L)的染毒组,在CO2培养箱中37℃分别培养24和2h,另设空白对照组和乙醇溶剂对照组,细胞经培养处理后采用单细胞凝胶电泳方法和微核实验方法检测DNA损伤作用,用傅立叶转换红外衰减全反射光谱技术(FT-IR-ATR)检测细胞损伤作用。结果与溶剂对照组比较,苯低、中和高剂量组的彗星细胞发生率、彗尾长度、微核率均增加,差异均有统计学意义(P<0.05,P<0.01),并且呈剂量-反应关系。与低浓度组比较,氢醌中和高浓度组彗星细胞发生率、彗星尾长度均升高(P<0.01);与空白对照组比较,氢醌低、中、高浓度组微核率升高(P<0.01),并且均呈剂量-反应关系。苯和氢醌ATR光谱图呈明显差异,用主成分统计分析,在三维空间旋转图上苯和氢醌点的分布与对照比较差异明显。结论高浓度苯和代谢产物氢醌均可诱导人淋巴细胞DNA单链断裂和染色体畸变,ATR光谱技术可作为早期细胞损伤的检测方法。
Objective To use heredity toxicity analysis to identify the adverse effects of benzene and metabolite hydroqui none. Methods Human lymphatic cells were isolated, cultured and then divided into five groups including negative control, solution control, low, moderate and high groups of benzene （0,0,20,300 and 5 000 μ/L） and four groups including negative control, low, moderate and high groups of hydroquinone （0,50,150 and 450 μmol/L） as well. After the treatment, lymphocyte DNA damage was detected by single cell gel electrophoresis and mieronucleus assay. Lymphocyte damage was detected by ATR microspectroscope. Results Compared with the solution control group, benzene groups＇comet rates, comet tail length and micronucleus rates increased respectively （P〈0.05）. Hydroquinone groups＇ micronucleus rates were higher compared with the control（P〈0.01 ）. Benzene and hydroquinone groups＇ ATR lines were different with control in ATR microspectroscope figure. Their line score plots showed different distribution in 3-D figure which was derived from principal component analysis. Conclusion High levels exposure of benzene and hydroquinone may cause human lymphocyte DNA damage. ATR microspectroscope can be used to detect early damage of cells.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2010年第6期485-487,F0003,共4页
Journal of Environment and Health
基金
广西科技厅资助课题(桂科功0472002-12)
关键词
空气污染
苯
氢醌
DNA损伤
红外衰减全反射
Air pollution
Benzene
Hydroquinone
DNA damage
Infrared attenusted total reflectance
