摘要
目的探讨免疫印迹法(IBT)检测ENA抗体在自身免疫性疾病中的诊断价值。方法应用IBT和免疫斑点技术对临床常见的自身免疫性疾病(143例)和31例健康者做对照组进行了抗ENA抗体检查,同时观察了IBT和免疫斑点技术与间接免疫荧光法检查抗核抗体(IFANA)结果的相关性。结果 143例送检标本IBT法检测ENA多肽抗体谱总阳性率为80.4%,且检出一种或一种以上抗ENA抗体,系统性红斑狼疮27例(88.89%),皮肤炎和多发性肌炎16例(62.50%),干燥综合征28例(92.86%),混合性结缔组织病12例(91.67%),类风湿关节炎60例(66.68%);斑点法检测ENA多肽抗体谱总阳性率为54.54%,且检出一种或一种以上抗ENA抗体,其中系统性红斑狼疮27例(51.85%),干燥综合征26例(85.71%),类风湿性关节炎26例(43.33%),结缔组织病8例(66.67%),皮肌炎及多发性肌炎5例(31.25%)。结论免疫印迹技术(IBT)敏感性好,特异度高,并对自身免疫性疾病的诊断、治疗及预后有重要的参考价值,而ANA检测仍可作为自身免疫性疾病筛查的常规指标。
Objective To investigate the diadynamic value of autoimmune diseases by determination of extractable nuclaer antibody（ENA）polypeptide autoantibody spectrum in serum.Methods Anti-ENA antibodyies were measured by the immunoblotting while antinuclear antibody were measured by the indirect immunofluorescence（IFANA）in 143 patients with 5 clinical common autoimmune diseases.Results The total positive rate of 143 specimens was 80.42%.The positive with one or more than one anti-ENA antibodyies which have already been checked out,in the systemic lupus erythematosus（SLE）,poly-derm atomyositis/derm atomyositis（ PM/DM ）,Sjogren syndrome （SS ）,mixed connective tissue disease （ MCTD ）,rheumatoid arthritis （RA ） were 27 （88.89%）,16（62.5%）,28（92.86%）,12（91.67%）,and 60（66.68%） respectively.The total positive rate of IFANA was 71%.Conclusion The IBT sensitirity and specificity is good,so that IBT is very important for the diagnosis,treatment and prognosis of autoimmune disease,while the detection method of ANA is more applicable in screening out the autoimmune disease.
出处
《国际检验医学杂志》
CAS
2010年第5期427-429,共3页
International Journal of Laboratory Medicine
基金
辽宁省教育厅基金资助项目(20060556)
关键词
自身免疫疾病
免疫印迹法
荧光抗体技术
间接
自身抗体
autoimmune diseases
immunoblotting
fluorescent antibody technique,indirect
Autoantibodies
