摘要
目的探讨抗核抗体（antinuclear antibodies，ANA）滴度与IgG，C3和C4的关系，以及不同滴度ANA与抗可提取的核抗原（extractable nuclear antigen，ENA）抗体阳性结果的相关性。方法对2010年5月～2012年6月间的1813例患者自身免疫检测结果进行分析，其中ANA和抗ENA抗体分别采用间接免疫荧光法和免疫印迹法检测，而IgG，C3和C4则采用免疫散射比浊法检测。按ANA检测结果将患者进行分组，ANA滴度1：100为第1组，ANA滴度1：160为第2组，ANA滴度1：320为第3组，ANA滴度1：640为第4组，ANA滴度1：1280为第5组，ANA滴度1：3200为第6组，ANA〉滴度1：3200为第7组，另在ANA滴度〈1：100中随机抽取120例为第8组（阴性组）。采用SPSS17．0统计学软件分析各组IgG，C3和C4的水平差异及各组抗ENA抗体的阳性率。结果随着ANA滴度的上升，IgG明显升高（F=31．19，P〈0．01），与阴性组比较差异均有统计学意义（t=2．57～6．66，P〈0．05）。当ANA滴度≥1：320时，C3，C4水平明显降低（F=42．15，9．57，P〈0．05），与阴性组比较差异均有统计学意义（t=3．58～12．21，3．58～9．69，P〈0．05）。随着ANA滴度的增高，抗ENA抗体阳性率逐渐升高（χ^2=86．67，P〈0．05），且各种抗ENA抗体的出现类型也有所不同，在1：100≤ANA滴度≤1；3200组中抗SS-A抗体阳性率最高，而在ANA滴度〉1：3200组中抗U1RNP抗体阳性率最高。结论血清IgG，C3和C4的水平与ANA滴度有一定的联系，而且ANA滴度与抗ENA抗体的出现类型也存在一定的相关性。临床应该重视ANA滴度在自身免疫性疾病中的临床价值。
Objective To explore the relationship between immunoglobulin G（IgG） and complement 3 （C3） and C4 with the titer of antinuclear antibodies （ANA）, and to investigate the correlation between the positive results of different items in the anti-extractable nuclear antigen （anti-ENA） with the titer of ANA. Methods The author analysed the results of autoimmune detection of 1813 patients from May 2010 to June 2012. ANA were tested by indirect immunofluorescence method,anti-ENA antibody were tested by ELISA method,IgG and C3,C4 were determined by immunoturbidimetry. According to the different titers,the patients were divided into several groups,the titer of ANA= 1 ： 100 as group 1 ,the titer of ANA= 1 ： 160 as group 2,the titer of ANA=1 ： 320 as group 3,the titer of ANA=1 ： 640 as group 4,the titer of ANA=1 ： 1 280 as group 5,the titer of ANA= 1 ： 3 200 as group 6, the titer of ANA〉3 200 as group 7,120 cases randomly selected from ANA〈1 ： 100 group as group 8（negative group）. The change of IgG and complement as well as the positive rates of the specific antibodies of anti-ENA were analyzed by SPSS17.0 software. Results When the titer of ANA≥ 1： 100,the levels of IgG were obviously higher than that in the negative group （F=31.19,P〈0.05）. When the titer of ANA≥1 ： 320,the levels of C3 and C4 were lower than that in the negative group（F= 42.15,9.57, P〈0.05）. Overall anti-ENA positive rates increased significantly with the titer of ANA （χ^2 = 86.67, P〈0. 05）, and the positive rates of various antibodies were different. Anti-SSA and anti-SSB were the most frequent at ANA titers of 1 ： 100 to 1 ： 3 200,while anti-U1RNP were the most frequent at ANA titers of 1 ： 3 200. Conclusion IgG and C3,C4 were related to the titer of ANA. The positive rates of the overall anti-ENA and some specific antibodies of anti-ENA were related to the titer of ANA. The clinician should pay attention to the value of ANA titer in autoimmune disease diagnosis.
出处
《现代检验医学杂志》
CAS
2013年第2期48-50,共3页
Journal of Modern Laboratory Medicine
基金
基金项目：国家临床重点专科建设项目资助[财社（20LO）305号].
