摘要
目的:研究胃癌组织中自发性细胞凋亡的发生情况及其临床意义。方法:采用TUNEL技术对53例胃癌组织中的凋亡细胞进行原位观察和比较。结果:胃癌组织自发性细胞凋亡指数平均为576%。胃癌细胞凋亡指数与患者年龄、性别、肿瘤大小、浸润深度无明显关系,与胃癌细胞分化程度、淋巴结转移及临床分期有关,高、中分化腺癌凋亡指数显著高于低分化腺癌(P<001),无淋巴结转移和临床Ⅰ、Ⅱ期胃癌凋亡指数显著高于有淋巴结转移和临床Ⅲ、Ⅳ期胃癌(P<005)。结论:提示胃癌组织自发性细胞凋亡与胃癌的某些生物学特性有密切的关系,细胞凋亡异常在胃癌发生发展过程中可能起重要作用。
To study the expression of spontaneous apoptosis in gastric carcinomas and its association with the biological characteristics of gastric carcinomas.Using the terminal deoxynucleotidyl transferase mediated nick end labelling(TUNEL)technique,We observed the apoptotic cells in situ in 53 gastric carcinoma tissues.The median apoptotic index in gastric carcinoma tissues was 5 76%.The apoptotic indexs had no correlation to the patient age,sex,tumor size,the depth of invasion;it was related to tumor cell differentiation,lymph node metastasis and Dukes' stages of tumors( P <0 01 and P <0 05).Apoptotic indexs in the cases of well/moderate differentiated,without lymph node metastasis or at stages Ⅰ and Ⅱ were significantly higher than in those poorly differentiated,with lymph node metastasis or stages Ⅲ and Ⅳ.The results suggest that the higher occurrence of apoptosis in well/moderate differentiated gastric carcinomas may reflect their slowgrowing nature,poorly differentiated gastric carcinomas and advanced stage gastric carcinomas may exist an escape mechanism from the process.Apoptosis may play an important role in the development of gastric carcinoma.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
1999年第2期98-100,共3页
Cancer Research on Prevention and Treatment
基金
军队医药卫生"九五"重点项目!96Z0 47
