摘要
将九合维生素丸样品溶于0.01 mol.L-1盐酸溶液中,经定量稀释并过滤后用于高效液相色谱分析。采用Dikma C18柱作固定相,并以不同比例配成的pH 3.5的0.1 mol.L-1磷酸二氢钾溶液(其中含1%乙腈)及乙腈-水(6+4)混合溶液作为流动相进行梯度淋洗,测定时用紫外检测法。用线性回归分析证明了所测6种水溶性维生素的线性关系良好,相关系数均大于0.999 9。其检出限(3S/N)在0.1～0.3 mg.L-1之间。对同一样品取6份进行测定,其测定值的相对标准偏差在1.5%～4.7%之间。VB1、VC、VB6、烟酰胺、泛酸钙、VB2的平均回收率依次为98.1%,108.7%,101.8%,99.7%,105.7%,92.7%。
Sample of multi-vitamin pills was dissolved in 0.01 mol·L-1 HCl,and the solution in a definite volume was filtered with 0.45 μm filtering membrane and used for HPLC analysis.The Dikma C18 column was used as stationary phase,and solutions of 0.1 mol·L-1 KH2PO4（adjusted to pH 3.5 with H3PO4） containing 1% by volume of acetonitrile mixed with acetonitrile-H2O（6＋4） solution in different ratios were used as mobile phase in the gradient elution.UV-detection was used in the determination.Linearity between values of peak area and concentration of 6 water soluble vitamins,namely vitamin B1,vitamin C,vitamin B6,nicotinamide,calcium pantothenate,and vitamin B2,were proved by linear regression analysis,giving correlation coefficients over 0.999 9.Detection limits（3S/N） found for these vitamins were in the range of 0.1 to 0.3 mg·L-1.Precision of the method was tested by 6 determination of the same sample,giving values of RSD′s（n=6） of 2.0%,2.2%,1.8%,2.8%,4.7% and 1.5% for the 6 vitamins respectively.Recovery was tested by standard addition method,results of average recovery found for the 6 vitamins were 98.1%,108.7%,101.8%,99.7%,105.7% and 92.7% respectively.
出处
《理化检验（化学分册）》
CAS
CSCD
北大核心
2010年第6期639-641,共3页
Physical Testing and Chemical Analysis(Part B:Chemical Analysis)
