摘要
目的研究再生障碍性贫血（AA）患者骨髓（BM）及外周血（PB）淋巴细胞及其活化相关分子的表达及临床意义。方法采用双色免疫荧光标记、流式细胞仪分析AA患者的BM和PB中淋巴细胞膜分子表达。结果AA患者BM和BP中CD8＋细胞增加，CD4／CD8比例下降，BM中CD25＋细胞和HLA-DR＋细胞增多，急性AA增加尤为显著（P（0．01），BM中CD16＋或CD56＋细胞也明显增多（P〈0．05）；双标记分析提示T细胞主要为CD8＋细胞；急性AA患者CD8＋-CD25＋细胞显著增多（P〈0．01），AA患者BM中淋巴细胞活化相关分子表达增多，尤其是4—1BB＋、CD95L＋和CD40L＋细胞显著增多（P〈0．01）。结论AA患者BM中淋巴细胞活化相关膜分子增多，是AA免疫功能异常及最终导致造血功能衰竭的原因之一。
Objective To investigate the phenotypes and expression of activated T cell membrane molecules on lymphoeytes in the Periperal blood （PB）and bone marrow （BM）of the patients with aplastie anemia. Methods Single and double-color immunofluoreseents were used to analyze the mononuelear cells from PB and BM of the AA patients by flow cytometry. Results The expression of CD8＋ increased and the ratio of CD4＋ / CD8＋ cell decreased significantly. The expression of CD25＋and HLA-DR＋ was increased in the BM, which was more significant in patients with serious AA（ P 〈 0.01 ） and the CD16＋ or CD56＋ positive cells also increased on BM. Double-color analysis showed that the most activated T eells were CD8＋ cells, the CD8＋ -CD25＋ lymphocytes increased signifieantly in the BM（P 〈 0. 01 ）. Co-stimulatory molecules which essential to activation of lymphocytes such as 4-1BB＋ , CD95L＋ and CD40L ＋ increased sigenficantly in the BM of AA patients. Conclusion The increase of co-stimulating molecules which was necessary for lymphocytes activation in the BM Of AA patients is one of main reasons to explain abnormal T cells activation and failure of hematopoiesis.
出处
《中国临床实用医学》
2010年第6期54-56,共3页
China Clinical Practical Medicine
