摘要
目的探讨ｐ５３，ｎｍ２３Ｈ１与原发性肝细胞癌（ＨＣＣ）发生发展的关系．方法运用原位分子杂交技术对４９例ＨＣＣ中ｐ５３和ｎｍ２３Ｈ１基因ｍＲＮＡ进行检测，并结合临床病理特征进行分析．结果ｐ５３ｍＲＮＡ杂交阳性２３例，占４６９％；ｐ５３ｍＲＮＡ过表达与肿瘤的肝内转移．包膜侵犯及Ｅｄｍｏｎｄｓｏｎ分级相关（Ｐ＜００５）；ｎｍ２３Ｈ１ｍＲＮＡ阳性表达２７例，占５５１％；ｎｍ２３Ｈ１ｍＲＮＡ表达与肿瘤肝内转移及ＴＮＭ分期呈负相关（Ｐ＜００５）；同时发现ｐ５３ｍＲＮＡ过表达和ｎｍ２３Ｈ１ｍＲＮＡ低表达在ＨＣＣ肝内转移中具有协同作用．结论ｐ５３和ｎｍ２３Ｈ１参与ＨＣＣ的发生发展，ｐ５３过表达及ｎｍ２３Ｈ１低表达提示ＨＣＣ肝内转移．
AIM To study the relationship between p53 and nm23 H1 mRNA expressions and progression of hepatocellular carcinoma (HCC). METHODS By using in situ hybridization, expressions of mRNA for p53 and nm23 H1 genes were detected in 49 cases of hepatocellular carcinoma, and analyzed in combination with clinical materials. RESULTS p53 mRNA was positive in 23 cases (46 9%). There was a significant correlation between the positivity of p53 mRNA and some pathological features, such as intrahepatic metastasis, infiltration to capsule and the degree of differentiation of HCC ( P <0 05). nm23 H1 mRNA was positive in 27 cases (55 1%). Expression of nm23 H1 mRNA was inversely correlated with intrahepatic metastasis and TNM stage. And there was synergism between overexpression of p53 mRNA and low expression of nm23 H1 mRNA in intrahepatic metastasis of HCC. CONCLUSION p53 and nm23 H1 are associated with progression of hepatocellular carcinoma, p53 overexpression and nm23 H1 lower expression may indicate the intrahepatic metastasis of HCC.
出处
《世界华人消化杂志》
CAS
1999年第1期40-42,共3页
World Chinese Journal of Digestology
关键词
肝肿瘤
信使RNA
P53基因
NM23-H1
基因表达
liver neoplasms
carcinoma, hepatocellular
RNA, messenger
p53 gene
nm23 H1 gene
gene expression
