摘要
目的:观察有肝毒性的栀子治疗小鼠急性肝损伤时会否加重肝损伤和健脾保肝方是否有助于栀子的药效。方法:c57bl雄性小鼠48只,随机分为正常组、模型组、栀子组、健脾保肝组、栀子合健脾保肝方组。正常组小鼠灌胃饮用水,其余小鼠均以相应药物灌胃,连续5天。第6天,除正常组小鼠,其余各组小鼠给予0.5%CCl4/橄榄油溶液,0.01mL·g-1腹腔注射,17h后处死。标本作病理和生化检测。结果:模型组肝细胞普遍变性,有散在的点状或灶状的坏死及炎细胞浸润;栀子组肝细胞损伤情况较模型组轻;健脾保肝组的变性肝细胞更少;栀子合健脾保肝组肝细胞的变性为4组中最轻者,未见肝细胞坏死。生化检测示栀子组和栀子合健脾保肝组的ALT活性明显较模型组轻[(597.89±364.17)、(466.90±222.26)vs(1008.30±460.73)U·L-1,P<0.05,P<0.01],栀子组、健脾保肝组、栀子合健脾保肝方组的AST活性均较模型组明显降低[(974.56±146.55)、(1033.13±137.01)和(721.33±127.2)vs(1238.7±260.51)IU·L-1,P<0.01或P<0.05]。结论:相当于临床成人剂量18g的栀子能够减轻CCl4所致的小鼠急性肝损伤;健脾保肝方与栀子配伍,增效或减毒,治疗急性肝损伤的作用更佳。
Objective：To investigate whether Gardenia which had the hepatotoxicity could induce more injury in mice with acute hepatic injury,and effects of Jianpi Baogan Recipe plus Gardenia.Methods：Forty-eight C57bl male mice were randomly divided into normal control group,model group,Gardenia group,Jianpi Baogan Recipe group and Jianpi Baogan Recipe plus Gardenia group.The mice in the treated groups were administrated with corresponding drugs for 5 days ;and the mice in the normal control group and untreated group were intragastricly administrated with drinking water.After 5-day pretreatment,the mice were intraperitoneally injected with 0.5% carbon tetrachloride and olive oil mixing solution（a dose of 0.01mL/ g）with exception of mice in the normal control group.Mice were sacrificed at 17 hours after injection and the samples were collected for histological and biochemical studies.Results ：HE straining showed that histological changes of liver tissues untreated group were obvious including hepatic cells degeneration,sporadic,punctiform or focal necrosis,infiltration of inflammatory cells.The cell injury in liver in Gardenia group was significantly lighter than those in the model group.Compared with the model group less hepatic cells degeneration was found in the Jianpi Baogan Recipe group,while the least hepatic cells degeneration was observed in Gardenia plus Jianpi Baogan Recipe group and there was no obviously neorobiosis.The activities of ALT in Gardenia group and Gardenia plus Jianpi Baogan Recipe group were significantly lower than those in the model group[（597.89±364.17）,（466.90±222.26）vs（1008.30±460.73）U/L,P0.05,P0.01].The activity of AST in the Gardenia group,the Jianpi Baogan Recipe group and Gardenia plus Jianpi Baogan Recipe group was also lower than those in model group obviously[（974.56±146.55）,（721.33±127.2）vs（1238.7±260.51）U/L,P0.01,P0.05].Conclusion ：Gardenia at concentration of 0.149g/mL,which is equal to dose of 18 g for person,can relieve the CCl4-induced liver
出处
《辽宁中医药大学学报》
CAS
2010年第6期65-68,共4页
Journal of Liaoning University of Traditional Chinese Medicine
基金
上海市重点学科建设资助项目(Y0302)
上海市教育委员会E-研究院建设计划项目(E03008)
上海市教育委员会重点学科(第五期)建设项目
上海高校创新团队建设项目(第一期)
关键词
中药
栀子
肝毒性
急性肝损伤
治疗
tradition Chinese medicine
gardenia
hepatic toxicity
prevention
