摘要
用甜瓜单性花即雌雄同株异花的多代自交系T912作母本,两性花即雄全同株多代自交系哈甜一号作父本,配制杂交F2群体,采用相关序列扩增多态性(SRAP)分子标记结合离体分组混合分析法(BSA)筛选与甜瓜单性花有关的基因标记,在64对组合引物中,找到1个能在单性花和两性花中扩增出特异条带的标记,并在F2群体中得到验证,命名为Sme1em5,计算出其遗传距离为8.9 cM。
The objective of this study was indentify SRAP marker for monoecious characteristic on pellicle melon. BSA（Bulked Segregant Analysis）was used to search for SRAP marker linked to monoecious gene, using F2 segreganting population derived from a cross of Tgl2（unisexual flower. ） and 1-4（hermaphrodite flower）. The subject found a maker smelem5 which was related to monoecious characteristic from 64 pairs of SRAP primers.
出处
《黑龙江农业科学》
2010年第6期1-4,共4页
Heilongjiang Agricultural Sciences
基金
哈尔滨市科学技术计划资助项目(GJ2007GG002536)
