摘要
以小鼠脾淋巴细胞为试验材料,将有丝分裂原刀豆蛋白A(ConA)或细菌脂多糖(LPS)与化疗药物5-氟尿嘧啶(5-Fu)联合使用建立了体外免疫抑制模型,并比较了多糖样品对5-Fu抑制淋巴细胞增殖的拮抗作用。结果表明,在供试样品中,F1-1[龙须菜(Gracilaria lemaneiformis)中分离多糖]和BW[沪农灵芝1号(Ganoderma lucidum)子实体中分离]对5-Fu产生的轻度抑制有完全拮抗作用,19号[沪农灵芝1号(G.lucidum)子实体中分离]样品刺激淋巴细胞增殖活性最好,但对5-Fu产生的抑制基本无拮抗作用,表明样品刺激淋巴细胞增殖的活性与拮抗化疗药物抑制淋巴细胞增殖的活性无正相关性。
National Research Center for Edible Fungi Biotechnology and Engineering; Key Laboratory of Applied Mycological Resources and Utilization,Ministry of Agriculture,the People＇s Republic of China; Key Laboratory of Agricultural Genetics and Breeding of Shanghai; Research Center of Natural Medicinal Resources,Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,Shanghai 201106,ChinaWe have developed an in vitro immunosuppression model based on the cell proliferation enhancing effects of the mitogens concanavalin A and lipopolysaccharide combined with the 5-fluorouracil （5-Fu）-induced inhibitory effect on mouse spleen lymphocyte proliferation. The model was used to compare the antagonistic effects of different polysaccharide preparations on 5-Fu-induced suppression of mouse spleen lymphocyte and mixed lymphocyte proliferation. Polysaccharide preparations F1-1 （from Gracilaria lemaneiformis） and BW （from Ganoderma lucidum） completely neutralized the mild inhibition of lymphocyte proliferation induced by 0.02 μg/mL 5-Fu. However,no positive correlation was found between the stimulating effect of a given polysaccharide on lymphocyte proliferation and the antagonistic effect of that polysaccharide on 5-Fu-induced suppression of lymphocyte proliferation. Thus,polysaccharide 19 （from G. lucidum） was most effective in stimulating lymphocyte proliferation but had no antagonistic effect on 5-Fu-induced suppression of lymphocyte proliferation.
出处
《食用菌学报》
北大核心
2010年第1期51-59,共9页
Acta Edulis Fungi
基金
上海市农业科学院院发基金"药用菌中拮抗化疗药物免疫抑制作用活性成分的筛选及作用机制研究"(编号:农科发2008-04)的部分研究内容~~
关键词
免疫抑制
化疗药物
多糖
Immunosuppression
chemotherapeutic agent
polysaccharide
