摘要
目的研究小鼠骨髓来源的未成熟CD11b+Gr-1+髓系前体细胞在急性炎性脂多糖刺激下动员释放,及其诱导分化成巨噬细胞样细胞后吞噬氧化型低密度脂蛋白形成泡沫细胞。方法小鼠腹腔注射脂多糖5μg/g体重,24h后采用流式细胞术分析骨髓,脾脏,和外周血中CD11b+Gr-1+髓系前体细胞,CD11b+Gr-1-单核细胞,和CD11b-Gr-1+粒细胞的百分比的变化。体外泡沫细胞形成实验取小鼠骨髓单个核细胞以DMEM培养基+胎牛血清培养,以粒细胞—巨噬细胞集落刺激因子诱导分化48h,加入100mg/L氧化型低密度脂蛋白孵育48h以形成脂质负荷泡沫细胞。油红O染色鉴定泡沫细胞。结果 (1)脂多糖腹腔注射可以显著促进CD11b+Gr-1+髓系前体细胞从骨髓动员并释放到外周组织;脾脏和循环中CD11b+Gr-1+髓系前体细胞和CD11b+Gr-1-单核细胞显著增加。(2)从骨髓分离的CD11b+Gr-1+髓系前体细胞以粒细胞—巨噬细胞集落刺激因子诱导分化后形成单核/巨噬细胞样细胞,继而加入氧化型低密度脂蛋白孵育,可以形成脂质负荷细胞(泡沫细胞)。油红O染色可见泡沫细胞的胞浆中有红染的大型脂滴,核被胞浆内脂滴挤压在一侧。结论急性炎性刺激脂多糖可以动员小鼠骨髓CD11b+Gr-1+髓系前体细胞的向循环和外周组织释放。CD11b+Gr-1+髓系前体细胞可被诱导分化并吞噬氧化型低密度脂蛋白形成泡沫细胞。
Aim To study the mouse bone marrow-derived immature CD11b^＋Gr-1^＋ myeloid progenitor cells in mice stimulated by acute inflammatory LPS, and induced to differentiate into macrophage-like cells phagocytized oxidized low density lipoprotein （ ox-LDL） to form into foam cells. Methods Mice were injected with lipopoly saccharide （LPS） 15μg/g weight, IP）, 24 hours later flow cytometry was used to analyze bone marrow, spleen, and peripheral blood CD11b^＋Gr-1^＋ myeloid precursor cells, CD11b＋Gr-1- mononuclear cells, and CD1b-Gr-1 ＋ granulocyte changed in proportion. Experimental in vitro formation of foam cells, mononuclear cells obtained from mice bone marrow were cultured in DMEM ＋ fetal bovine serum （FBS） medium, co-cultured with granulocyte-macrophage colony-stimulating factor （GM-CSF） for 48 hours to differentiate into monocyte / macrophage-like cells, then co-cultured with ox-LDL（ 100 mg/L ） for 48 hours to form lipid burdened foam cells. Oil red O staining identified foam cells. Results （ 1 ） LPS intraperitoneal injection can promote the CD11b ＋ Gr-1＋ myeloid precursor cells from bone marrow to mohilizate significantly and to release into the peripheral tissue; the amount of CD11b^＋Gr-1^＋ myeloid precursor cells and CD11 b ＋ Gr-1 - mononuelear cells in spleen and circulating pool increased significantly. （2 ）CD11b^＋Gr-1^＋ myeloid precursor cells which were isola- ted from bone marrow were co-cultured with GM-CSF differentiated into monocyte / macrophage-like cells, and then were incubated with ox-LDL to form lipid burdened cells （ foam cells）. Oil red O staining showed the cytoplasm of foam cells with red-dyed, large lipid droplets, nuclear were squeezed by the lipid droplets to the comer. Conclusion Acute inflammatory stimulus LPS can mobilize the mouse bone marrow CD11b^＋Gr-1^＋ myeloid precursor cells to release to the circu- lation and peripheral tissue. CD11b^＋Gr-1^＋ myeloid precursor cells can be induced to differentiate and phagocytize ox
出处
《中国动脉硬化杂志》
CAS
CSCD
北大核心
2010年第3期189-192,共4页
Chinese Journal of Arteriosclerosis
基金
江苏省科教兴卫重点工程(RC2007104)
