摘要
目的研制检测水样品中镉离子残留的胶体金免疫层析快速检测试纸条。方法采用免疫竞争法,将抗Cd2+-EDTA单克隆抗体-胶体金复合物包被于胶体金结合垫上,并将人工合成的Cd-iEDTA-BSA检测抗原包被在硝酸纤维素薄膜表面,作为检测线(T线),3～5min后,根据颜色直观显示检测结果。对试纸条进行灵敏度、特异性和稳定性验证,并检测添标水样。结果制备的试纸条对镉离子的最低检测限为100ng/ml;除了与Hg2+-EDTA有交叉反应外,与Fe3+、Pb2+、Cu2+等类似物无交叉反应;试纸条在常温下放置8周稳定性良好;检测添标水样的结果与ICP-AES的检测结果一致。结论胶体金免疫层析法操作便捷,稳定可靠,可作为水样中重金属镉离子残留现场检测和监控的有效手段。
Objective To prepare a colloidal gold immunochromatography strip for rapid determination of residual cadmium ion in water samples.Methods Competitive assay was adopted.Colloidal gold binding cushion was coated with anti-Cd2＋-EDTA monoclonal antibody-colloidal gold complex,and cellulose nitrate film with synthetic Cd-iEDTA-BSA to form the test（T）band.The determination result was observed 3～5 min later and judged according to the color of T band.The prepared strip was verified for sensitivity,specificity and stability,and used for determination of water samples containing cadmium standard.Results The minimum detection limit of cadmium ion by the prepared strip was 100 ng /ml.Except that with Hg2＋-EDTA,no cross reactions with other metal ion such as Fe3＋,Pb2＋ and Cu2＋ were observed.The strip showed good stability after storage at room temperature for 8 weeks.The determination result of water samples containing cadmium standard by the prepared strip was consistent with that by ICP-AES.Conclusion Colloidal gold immunochromatography is easy to handle,stable and reliable,which may be used as an effective tool for field determination and monitoring of cadmium ion in water samples.
出处
《中国生物制品学杂志》
CAS
CSCD
2010年第5期529-532,共4页
Chinese Journal of Biologicals
基金
国家"863"计划(2008AA10Z427)
天河区科技计划项目(085G079)资助
关键词
镉离子
EDTA
胶体金
免疫层析法
单克隆抗体
Cadmium ion
EDTA
Colloidal gold
Immunochromatography
Monoclonal antibody
