摘要
目的分析河南省9株狂犬病毒的核蛋白基因序列,探讨狂犬病毒流行株毒力与致病性的可能变异。方法以RT-nested-PCR扩增9株2006年12月分离于河南省信阳市的狂犬病毒街株,经纯化、克隆、测序后获得9条N基因全长序列,采用生物信息学软件对N基因序列进行分析。结果9株狂犬病毒核蛋白在核苷酸及氨基酸水平上彼此的同源性分别为97.5%～99.3%和98.4%～99.8%;病毒与CTN疫苗的核苷酸序列同源性最高,为88.9%～90.1%,氨基酸序列同源性为97.6%～98.0%;与其他疫苗株相比,9株病毒与其核苷酸及氨基酸同源性范围分别为84.4%～87.9%和94.2%～97.3%;与已知的基因1型狂犬病毒比较,9株病毒核蛋白氨基酸序列发生了若干位点的取代。结论9株河南省狂犬病毒流行株均属基因1型,其核蛋白在基因的核苷酸及推导的氨基酸水平上均有变异,这些变异可能影响疫苗对流行株所致狂犬病的保护效果。
Objective To analyze 9 nucleoprotein gene sequences of rabies viruses circulating in Henan province,China,and to explore the probable mutant on their pathogenicity and virulence. Methods N gene sequences of 9 rabies viruses derived from Henan province were amplified by RT-nested-PCR,and then were cloned and sequenced.Bioinformatics software was used to determine the genetic characteristics of these rabies viruses. Results The sequences of N gene of nine Henan isolates were identical,with homogeny between 97.5%-99.3% and 98.4%-99.8% on nucleotide and deduced amino acid level,respectively.They were much more homogeneous with CTN than with other vaccine strains,with the identities being 88.9%-90.1% and 97.6%-98.0% on nucleotide and amino acid,respectively,while the identities between them and other vaccine strains were 84.4%-87.9% and 94.2%-97.3%.There were several amino acid substitutions in the nucleoprotein of 9 Henan isolates,compared with the known genotype 1 isolates. Conclusions The rabies viruses derived from Henan province were still genotype 1.These isolates of rabies virus were diverged from the vaccines in both gene sequences and deduced amino acid sequences,and these divergences might result in the inefficiency of the vaccines.
出处
《复旦学报（医学版）》
CAS
CSCD
北大核心
2010年第3期331-335,共5页
Fudan University Journal of Medical Sciences
关键词
狂犬病毒
N基因
遗传特征
河南省
rabies virus
N gene
genetic characteristics
Henan province
