摘要
为了解传染性法氏囊病病毒(IBDV)HLJ-0504株的分子生物学特征,本研究利用融合PCR技术获得HLJ-0504株全基因组序列。核苷酸和推导氨基酸序列遗传演化分析发现,HLJ-0504A节段位于IBDV超强毒的分枝上,而B节段则介于超强毒株和减毒株之间,形成一个独立分枝,属于一株新的自然重组病毒。VP2抗原性预测分析表明,HLJ-0504株VP2第Ⅰ亲水区中的氨基酸发生突变(D212N),该突变可能导致了HLJ-0504株抗原性发生漂变。本实验结果为深入研究IBDV的分子特征奠定了基础。
The full length genomes of very virulent infectious bursal disease virus（vvIBDV） HLJ-0504 isolate was cloned by fusion PCR and the DNA fragments of segment A and B were sequenced.Genetic evolution analysis showed that the segment A of the HLJ-0504 isolate was located on the branch of vvIBDV,however the segment B formed an independent branch between attenuated and vvIBDV strains,indicating that natural reassortment might have occurred between different IBDV stains.There was an amino acid change from D to N at position 212 in the first hydrophilic region of VP2,which could result in the drift of the antigenicity of HLJ-0504.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2010年第5期401-404,共4页
Chinese Journal of Preventive Veterinary Medicine
基金
国家973项目(2005CB523202)
中国博士后科学基金(20080440921)
黑龙江省博士后资助经费(LBH-Z08016)
