摘要
目的:观察组胺对穴位处肥大细胞的激活作用,以及其引起的类似针刺的镇痛效应,讨论穴位注射疗法可能存在的机制。方法:SD大鼠60只,随机分为空白对照组、模型组、生理盐水对照组、手针组和组胺注射组。大鼠左踝关节腔注射完全弗氏佐剂0.05 mL制作佐剂性关节炎模型。测量各组痛阈变化,并对穴位组织进行组织学观察,统计肥大细胞脱颗粒率。比较在"足三里"穴位注射100μg/mL的组胺溶液50μL和手针刺激20 min下的镇痛效应的差异性。结果:组胺注射和手针针刺,都能提高大鼠的痛阈达到镇痛效果,与模型组相比治疗后痛阈都有显著提高(P<0.01)。组胺引起的肥大细胞脱颗粒率与生理盐水相比明显增高(P<0.01),与针刺相比差异无统计学意义(P>0.05)。结论:在佐剂性关节炎大鼠模型中,组胺可以引起穴位处肥大细胞脱颗粒,产生与针刺类似的镇痛效应。以刺激肥大细胞为目的的新型穴位注射疗法存在可行性。
Objective To observe the activation effect of histamine on mast cells in the acupoint area and its analgesic effects similar to acupuncture stimulation, so as to reveal the underlying mechanism of acupoint injection therapy. Methods A total of 60 SD rats were divided into control, model, normal saline （NS）, histamine-injection and acupuncture groups （12 rats/ group）. Acute adjuvant-induced-arthritis （AIA） model was duplicated by injection of complete Freunds＇ Adjuvant （0.05 mL） into the left ankle articular cavity. For rats of saline and histamine groups, normal saline （50 μL） and histamine （50 μL, 100 μg/mL） were injected into the left ＂Zusanli＂ （ST 36） acupoint area. For rats of acupuncture group, manual acupuncture （lifting-thrusting and twisting needle intermittently） was applied to ST 36 for 20 min. Paw withdrawal （pain threshold, PT） was detected by radiant heat irradiation of the hindpaw. Skin and muscle tissues of ST 36 area were sampled to be fixed in formalin, then, sliced （5 μm）, and stained with Toluidine Blue, separately, followed by counting the degranulation rate of mast cells under microscope. Results Compared with normal control group and pre-modeling, PT of model, NS, acupuncture and histamine groups decreased significantly （P〈0.01）, while in comparison with model group, PT of acupuncture and histamine groups increased considerably （P〈0.01）, suggesting an induction of analgesic effect after acupuncture and histamine injection. The degranulation rates of acupuncture and histamine groups were remarkably higher than those of model and NS groups （ P〈0.01 ）, suggesting an activation of mast cells following regional acupuncture stimulation and histamine injection. Conclusion Both manual acupuncture and histamine injection of ST 36 can relieve pain in AIA rats, which may be closely related to its effect in activating mast cells in acupoint area. It suggests that the mast cells are a new target for acupoint injection therapy.
出处
《针刺研究》
CAS
CSCD
北大核心
2010年第2期99-103,共5页
Acupuncture Research
基金
上海市重点学科建设资助项目(No.B112
S30340
T0302)
上海市科技发展基金(No.06DZ19732
064319053
07DZ19722)
上海市科委科研平台建设专项(No.2008DZ1973503)
国家重点基础研究发展计划("973"项目
No.2005CB523306)
关键词
急性关节炎
针刺
穴位注射
镇痛
组胺
肥大细胞
Acute arthritis
Acupuncture
Acupoint injection
Analgesic effect
Histamine
Mast cells
