摘要
目的建立烫伤合剂中盐酸小檗碱的含量测定方法。方法色谱柱为Inertsil C8-3柱（4.6 mm×250 mm,5μm）,流动相为0.05 mol.L^-1磷酸二氢钠溶液-乙腈-磷酸（70∶30∶0.08）;流速为1 mL.min^-1,柱温：35℃,检测波长为260 nm。结果盐酸小檗碱在5.28-52.8 mg.L^-1内峰面积与浓度呈良好的线性关系,Y=2.45×10^4X＋4.91×10^3（r=0.999 4）,平均回收率为100.30%,RSD为1.24%（n=6）。结论方法简便、准确、重复性好,可作为控制产品质量的定量依据。
OBJECTIVE To determine the content of berberine hydrochloride in mixture for burn and scald.METHODS The content of berberine hydrochloride in mixture for burn and scald was determined by HPLC with Inertsil C8-3（4.6 mm× 250 mm,5 μm） column.The mobile phase was 0.05 mol.L^-1 sodium dihydrogen phosphate solution-acetonitrile-phosphoric（70 ∶ 30 ∶ 0.08） with a flow rate of 1 mL.min^-1,the columm temperature was set at 35 ℃ and the wavelength of the detector was set at 260 nm.RESULTS The linear range was 5.28-52.8 mg.L^-1 and the correlation cofficient was 0.999 4.The average recovery and the RSD were 100.30% and 1.24%（n=6）.CONCLUSION The method is simple,accurate and repeatable.It is proved to be suitable for the quality control of mixture for burn and scald.
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2010年第5期439-441,共3页
Chinese Journal of Modern Applied Pharmacy
