摘要
目的 探讨阻断缝隙连接通讯对兔颅脑爆震伤后下丘脑神经元凋亡的影响. 方法 将48只白兔按照随机数字表法分为对照组和实验组,对照组在左侧脑室注入生理盐水1 mL,实验组则在左侧脑室注入缝隙连接特异性阻断剂辛醇1 mL,2h后用纸雷管爆炸制作兔颅脑爆震伤模型.并于伤后1 d、3 d、7 d分3组处死,每组8只.采用Western-blot定量分析法、免疫组化及RT-PCR方法 ,分别观察脑损伤后下丘脑缝隙连接蛋白43（Cx43）表达以及凋亡特异性蛋白Caspase-3的变化. 结果 实验组下丘脑Cx43的表达随时间推移呈上升趋势,3 d达到高峰,但同一时间点的表达均低于对照组,差异均有统计学意义（P〈0.05）;实验组伤后同一时间点Caspase-3的表达均明显低于对照组,差异均有统计学意义（P〈0.05）. 结论 阻断缝隙连接通讯可以减少脑外伤后下丘脑神经元凋亡的发生率,Cx43参与了下丘脑神经元凋亡信息传导的过程.
Objective To explore the effect of blocking of gap junction communication on neuronal apoptosis in rabbit hypothalamus after explosive brain injury. Methods Forty-eight rabbits were equally randomized into control group and experimental group. Octanol, a gap junction blocker, was injected through the left ventricle to block the gap junction communication in the experimental group and physiological saline in the control group instead. Rabbit models with explosive brain injury were induced by paper detonators 2 h after that. One, 3 and 7 d after the inducement, these rabbit models were sacrificed and thus, they were subdivided into 3 groups （n=8）. The protein expression of gap junctional connexins 43 （Cx43） was observed by Western blotting and caspase-3 protein expressions in the hypothalamus were detected by immunohistochemical staining and RT-PCR. Results The protein expression of Cx43 in the experimental group was significantly lower than that in the control group at all time points （P〈0.05）, and that in the experimental group reached the peak level 3 d after the brain injury.The expressions of caspase-3 in the experimental group were significantly lower than those in the control group after the injury （P〈0.05）. Conclusion Blocking of gap junction communication can reduce the incidence rate of neuronal apoptosis in the hypothalamus after brain injury, and Cx43 might play an important regulation role in this process.
出处
《中华神经医学杂志》
CAS
CSCD
北大核心
2010年第5期460-464,共5页
Chinese Journal of Neuromedicine
基金
南京军区联勤部卫生部十一五重点基金项目（2006z19）
