摘要
目的在视觉发育可塑性关键期终止前后,观察软骨素酶ABC(chondroitinase ABC,ChABC)降解LongEvans大鼠视皮层硫化软骨素粘多糖(chondroitin sulfate proteoglycans,CSPGs)后闪光视觉诱发电位(flash visual evoked potential,F-VEP)的变化。方法对出生后21、35、49d正常组及同时间点ChABC酶视皮层灌注组大鼠进行F-VEP检查。结果正常组大鼠21、35、49d,F-VEP主波的潜时逐渐缩短,分别为[(63.5±10.1),(50.8±6.4),(44.9±6.3)ms,P<0.05],波峰值由21d的(15.7±3.8)ms增加至35d的(26.6±4.0)ms(P<0.05),49d较35d稍降低。35、49dChABC酶视皮层灌注组大鼠F-VEP主波的潜时分别为(62.5±8.0),(58.4±7.6)ms,较同时间点正常组明显延长(P<0.05),主波振幅两组间各时间点无明显差异(P>0.05)。结论在视觉发育可塑性关键期终止前后,降解大鼠视皮层CSPGs影响了F-VEP的发育变化。
Objective To investigate the effects of chondroitin sulfate proteoglycans （CSPGs） degradation induced by chondroitinase ABC （ChABC） on the rat flash visual evoked potential （F-VEP） around the end of the critical period of visual development.Methods A total of 30 Long Evans rats were randomly divided into 3 groups according to their age of 14,21 and 35 d.Then in each group,5 animals served as CSPGs degradation model induced by ChABC microinjection into 5 sites of the rat cortex [750 nL （48 U/ml） for 1 site,for 2 times at a 3-day interval],and the other 5 rats served as normal control and received normal saline microinjection.Immunofluorescence microscopy and laser scanning confocal microscopy were used to determine the establishment of animal model.F-VEPs were evaluated in 7 d after first microinjection.Results In the normal rats,the latency of F-VEP main wave became shorter with the increase of their age,the number was [（63.5±10.1）,（50.8±6.4）,（44.9±6.3） ms,P0.05],whereas the amplitude of F-VEP main wave was first increased from （15.7±3.8） ms to （26.6±4.0） ms at early postnatal days （P0.05） and decreased slightly at postnatal day 49.In the ChABC treated rats,the latency of F-VEP main wave became longer than that of normal rat on postnatal day 35 and 49,the number was （62.5±8.0）,（58.4±7.6）ms （P0.05）.But there was no obvious change of the F-VEP amplitude between the normal rats and ChABC treated rats.Conclusion Around the end of the critical period of visual development,CSPGs degradation has an effect on the development of rat F-VEPs.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2010年第10期1031-1033,共3页
Journal of Third Military Medical University
基金
国家自然科学基金(30772350)~~
关键词
硫化软骨素粘多糖
闪光视觉诱发电位
视皮层
视觉发育
chondroitin sulfate proteoglycans
flash visual evoked potential
visual cortex
visual development
