摘要
目的探讨不同时间亚叶酸钙(CF)对大剂量甲氨蝶呤(HD-MTX)化疗白血病小鼠肠黏膜的保护作用。方法建立白血病小鼠模型60只,随机分为5组,各12只。A组:正常对照组;B、C、D组:实验组,分别于腹腔注射HD-MTX后24、36、42h予以CF解救;E组:空白对照组,不予CF解救,余同实验组。观察各组小鼠病死率、一般情况及空肠大体形态,标本测定肠黏膜绒毛长度和隐窝深度。结果 A组无腹泻,B、C、D、E组第2天开始出现腹泻,第3天全部出现腹泻,其中D、E组腹泻严重。A组肠壁无充血水肿,B、C、D、E组肠壁充血水肿变薄,其中E组最重。A组无死亡,C、D组各死亡4只,B组死亡3只,E组死亡8只。B、C、D、E组绒毛高度和隐窝凹陷深度与A组比较,差异有统计学意义(P<0.05);B、C、D组绒毛高度与隐窝凹陷深度与E组比较,差异有统计学意义(P<0.05);B、C、D组间绒毛高度与隐窝凹陷深度比较,差异无统计学意义(P>0.05)。结论 HD-MTX化疗后42h开始应用CF解毒疗效好,不良反应小,安全可行。
Objective To explore different time of calcium folinate（CF） for p-rotecting enteral mucosa after chemo-therapy of high-dose methotrexate（HD-MTX） in leukemic mouse. Methods To take 60 leukemic white mouses and divide them into 5 groups,each of 12 cases. Group A：normal control group. Group B,C and D：Test groups,and rescuce them with CF after their HD-MTX injection of 24 hours,36 hours and 42 hours on an empty stomach. Group E：the blank control group with-out the rescue of CF. Observed the death rate,general condition and general shape of the empty the small intestine,length of the villus and depth of recesses in each group. Results Group A：No diarrhea. Group B,C,D and E：Some bagin to have a diarrhea from the second day and all in the third day. Among them Group D and E were more serious. Group A had no congestion and edema on the intetinal wall,B,C,D and E：The intestinal wall become thinner because of congestion and edema. Group B and E were the most serious. No white mouse died in Group one,four died in group B and C individully,three in group 3 and eight in group E. The intestinal mucosa villus length in Group B,C,D and E. Coparing with Group A has a statistical significance（P 0. 05）;The differences between B,C,D and E also had a statistical significance（P 0. 05）,the differences between B,C and D had not significance（P 0. 05）. Conclusion After 42 hours the completion of chemotherapy was the optimal time for CF rescue therapy with higher efficacy and less toxicity and side effects.
出处
《临床合理用药杂志》
2010年第9期46-48,共3页
Chinese Journal of Clinical Rational Drug Use
