摘要
利用电脉冲将质粒pHT3101和pHE－4D分别转入了大肠杆菌和苏云金杆菌，结果显示大肠杆菌DNA的转化率（转化子）为104～105·μg－1，苏云金杆菌的DNA的转化率（转化子）为102～103·μg－1．同时利用该法消除了Escherichiacoli菌株TG1和Bt菌株ISP78／11中的pHT3101质粒，消除率分别为88．6％和66．4％．比较了转化和质粒消除的条件并构建了一个工程菌．
Electroporation is one of the most effective methods for transferring DNA into cells, and appears to be applicable in a wide range of bacterial species. Plasmids pHT3101 and pHE-4D were int-roduced into Escherichia coli and Bacillus thuringiensis (Bt) by electroporation, the transformation efficiencies were around 104-105 and 102-103 transformants per μg DNA for E. coli and Bt, respectively. Electroporation was also used successfully in curing plasmid pHT3101 from E. coli TG1 and Bt ISP78/11 at frequencies of 88.6 % and 66.4%,respectively. The conditions of transformation and plasmid curing were discussed and an engineering strain was constructed by electroporation.
出处
《福建农业大学学报》
CSCD
1999年第1期43-46,共4页
Journal of Fujian Agricultural University
基金
国家科委资助!96-C01-02-01
