摘要
目的:建立HPLC法测定那他霉素的含量和有关物质。方法:采用Diamonsil C18色谱柱(150mm×4.6mm,5μm);流动相:缓冲液(醋酸铵3.0g和氯化铵1.0g,加水760mL溶解并混匀)-乙腈-四氢呋喃(76:24:0.5);流速:1.5mL.min-1;检测波长:303nm;柱温:室温。结果:那他霉素浓度在100.136～1001.36μg.mL-1范围内线性关系良好(r=0.9999),最低检测限(S/N=3)为0.4ng。结论:本方法简便、灵敏、专属,重复性好,结果准确可靠,可用于测定那他霉素含量和有关物质。
Objective：To establish an HPLC method for the determination of content and related substances of natamycin.Methods：The analysis was carried out with Diamonsil C18 column（150 mm×4.6 mm,5 μm）,using buffer（measure 3.0 g ammonium acetate and 1.0 g ammonium chloride,dilute with water to 760 mL）-acetonitrile-tetrahydrofuran（76：24：0.5） as mobile phase.The flow rate was 1.5 mL·min-1,the detection wavelength was set at 303 nm and the column temperature was room temperature.Results：The calibration curve for natamycin was linear in the rang of 100.136-1001.36 μg·mL-1.The detection limit（S/N=3） was 0.4 ng.Conclusion：The method is simple,sensitive,specific,reproducible,accurate and suitable for examination of the content and related substances of natamycin.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2010年第5期929-932,共4页
Chinese Journal of Pharmaceutical Analysis
