摘要
从猕猴桃大田根际土壤和青海牦牛粪中分离到86株芽孢杆菌。平板对峙试验发现其中8个菌株对猕猴桃细菌性叶枯病病原菌Pseudomonas syringae var. syringae的拮抗性能明显。经管碟法复筛,得到2株对病原菌具有显著拮抗作用的芽孢杆菌M24和Q13。田间试验表明,发酵液(109cfu/ml)稀释100倍时喷施,菌株M24和Q13对猕猴桃细菌性叶枯病的防效分别达到85.9%和80.6%,均显著高于95%CT杀菌剂的60.5%防效。2个菌株均具有良好的田间应用潜力。
Eighty-six bacterial strains were isolated from rhizosphere soil of kiwifruit and dung of Qinghai wild Yak.Dual culture experiments revealed that 8 bacterial strains were of obvious antibacterial activities against the pathogen of kiwifruit bacterial leaf blight disease.Among them,strain M24 and Q13 were much more effective than the others in secondary screen through cylinder-plate method.Results of field trails showed that the control effect of M24 and Q13 reached to 85.9% and 80.6%,respectively,when their fermentation broth was diluted 100 times,significantly higher than the control effect（60.5%） of 95% CT,a chemical bactericide.Both M24 and Q13 presented excellent biocontrol potential in fields.
出处
《中国生物防治》
CSCD
北大核心
2010年第2期235-239,共5页
Chinese Journal of Biological Control
基金
陕西省科学院重点项目(2008K-01)
