摘要
目的：ＳＥＢ诱导增殖的外周血Ｔ细胞是否具有正常Ｔ细胞的功能。方法：用超抗原刺激淋巴细胞生长；用流式细胞测定技术检查细胞表面ＣＤ４，ＣＤ８，ＣＤ２８，ＴＣＤ１６的抗原表达量；加入同源，异源抗原检查自身反应性。结果：ＳＥＢ能诱导外周血ＣＤ４＋Ｔ细胞的增殖。随ＣＤ４＋Ｔ细胞的增加，ＣＤ２８分子表达上升。外周增殖的ＣＤ４＋Ｔ细胞与胸腺内分化的Ｔ细胞相同，９７％以上的ＣＤ４＋Ｔ细胞都表达ＣＤ２８分子。但对ＣＤ１６阳性的抗原提呈细胞的依赖性下降，并显示对自身抗原的识别能力增强。结论：外周活化的ＣＤ４＋Ｔ细胞与胸腺内分化的ＣＤ４＋Ｔ细胞功能不同。
Objective:We examined the characteristics of human peripheral blood T cells activation by superantigen B(SEB), and try to know if the proliferous T cells are as same as entrothymic T cells.Methods:We analyzed CD4, CD8, CD16 and CD28 molecular expression and autoimmunity of proliferous T cells by flow cytometry and cell culture. Results:Most of CD4 + T cells expressed CD28 molecular The CD4 +CD8 +T cells are different from extrothymic T cells. The dependence for T cells to APC exhibited decrease and showed autoimmunity against to isogenous antigen. Conclusion:The function of CD4 +T cells activation in peripheral blood are different from the function of extrothymic T cells.
出处
《军医进修学院学报》
CAS
1999年第1期9-11,共3页
Academic Journal of Pla Postgraduate Medical School
基金
国家科学技术部基金
