摘要
目的 了解Akt和磷酸化Akt1（p-Akt1）蛋白在胰腺癌组织中的表达情况,探讨其临床意义.方法 应用免疫组织化学法检测74例胰腺癌组织、10例正常胰腺组织中Akt和p-Akt1蛋白的表达,并分析其与临床病理学特征及预后的关系.结果 Akt和p-Akt1蛋白在胰腺癌组织中的阳性表达率分别为87.8%和83.8%,而正常胰腺组织均阴性表达,相差非常显著（P〈0.05）.癌组织中Akt和p-Akt1蛋白的表达呈正相关性（r=0.274,P=0.018）.Akt和p-Akt1蛋白表达与年龄、性别、肿瘤生长部位、肿瘤大小、病理学分级、淋巴结转移、临床分期及神经浸润均无显著相关性（P〉0.05）,但p-Akt1蛋白高表达与病理学T分期及TNM分期相关（P=0.002）.Akt和p-Akt1蛋白高表达者的中位生存期分别为（16.0±5.7）个月和（23.0±5.5）个月,明显高于低表达者的（9.3±0.2）个月和（11.1±1.8）个月（P分别为0.007和0.004）.结论 胰腺癌组织中p-Akt1蛋白表达的程度与良性预后有关,检测胰腺癌中p-Akt1蛋白的表达具有一定的临床意义.
Objective To investigate the expression of Akt and phosphoryled Akt （p-Akt1） protein in pancreatic carcinoma and to determine the clinical significance. Methods In 74 cases of pancreatic carcinoma and 10 cases of normal pancreatic tissue samples, the expression of Akt and p-Akt1 were detected by immunohistochemical method, and the its relationship with clinicopathologic characteristics and prognosis were analyzed. Results The positive expression rate of Akt and p-Akt1 in pancreatic carcinoma were 87.8% and 83.8% respectively, while there was no expression of Akt and p-Akt1 in normal pancreatic tissue, and the difference was statistically significant （p 〈 0.05）. There was a positive correlation between the expression of Akt and p-Akt1 in pancreatic carcinoma （r =0.274, P =0. 018）. The expression of Akt and p-Akt1 was not significantly associated with the age, sex, location, size, pathology stages, lymph nodes metastasis, clinical stages and nerve invasion of the tumor （P 〉0.05）. But the higher expression of p-Akt1 was associated with T stages and TNM staging （p =0. 002）. Patients with high intensity of Akt and p-Akt1 expression showed a significantly longer median survival time [（16.0 ± 5.7） month and （23.0 ± 5.5） month, respectively]than those with low intensity expression [（9.3 ± 0.2） month and （11.1 ± 1.8） month （P = 0. 007 and P = 0.004） respectively]. Conclusions p-Akt1 expression is a significant positive prognostic factor for pancreaticcarcinoma and detection of p-Akt1 expression may be of clinical value.
出处
《中华胰腺病杂志》
CAS
2010年第2期128-130,共3页
Chinese Journal of Pancreatology
