摘要
目的:探讨MAPK反义寡核苷酸对肝癌细胞恶性表型的逆转作用。方法:用脂质体转染法将MAPK反义寡核苷酸导入肝癌细胞株SMMC-7721中,通过细胞活力、蛋白质含量、集落形成和DNA合成的变化检测MAPK反义寡核苷酸对肝癌恶性表型的逆转作用。结果:MAPK反义寡核苷酸可明显抑制细胞活力、蛋白质含量、集落形成和DNA合成,其抑制率分别为52.6%、50.5%、54.9%和47.5%,与对照组比较差异有显著性(P<0.01)。结论:MAPK反义寡核苷酸可逆转SMMC-7721细胞恶性表型,也为肝癌治疗指出了新方向。
Objective To study the reversal effect of MAPK antisense oligodeoxynucleotide （ASO） on SMMC-7721 ceils. Methods After MAPK ASO was introduced into SMMC-7721 cells by liposomal transfection,the effect was evaluated by ceil viability ,protein content,colony formation and DNA synthesis. Results The cell viability, protein content, colony formation and DNA synthesis were signiilcanfly inhibited by MAPK ASO, the inhibitory rate was 52. 6%, 50.5% , 54. 9% and 47.5% respectively（ P 〈 0.01） . Conclusion MAPK ASO can reverse the malignant phenotype of SMMC-7721 ceils, which may be use as a new approach in the treatment of liver cancer.
出处
《实用医学杂志》
CAS
2005年第19期2116-2118,共3页
The Journal of Practical Medicine
