摘要
目的观察超声消融术结合化疗卡铂对Ｗａｌｋｅｒ－２５６和ＳＭＭＣ－７７２１癌细胞的杀伤作用，以期排除癌栓消融后癌细胞播散转移可能。方法应用ＭＴＴ法测定不同剂量超声结合不同浓度卡铂消融Ｗａｌｋｅｒ－２５６和ＳＭＭＣ－７７２１癌细胞的细胞毒作用，另将处理过的Ｗａｌｋｅｒ－２５６细胞分别行ＳＤ大鼠皮下接种和门静脉灌注，观察其肿瘤生长情况。超声消融剂量：４２ＫＨｚ，０．２２Ｗ和０．１５Ｗ。卡铂浓度为０～１００μｇ／ｍｌ。结果超声消融能显著增强化疗卡铂药物对Ｗａｌｋｅｒ－２５６和ＳＭＭＣ－７７２１的杀伤作用，药物剂量越大，超声能量越高，杀伤作用越强。先化疗再超声比先超声再化疗有效。体内实验证实，超声消融（４２ＫＨｚ，０．２２Ｗ）结合卡铂（６０μｇ／ｍｌ）处理Ｗａｌｋｅｒ－２５６后，大鼠皮下和肝内肿瘤发生率均为０。结论超声消融结合化疗可有效防止癌细胞远处播散转移。
Objective To study the cytotoxicity of ultrasonic disruption combined with carboplatin on cellsuspensions of Walker-256 and SMMC-7721 tumor cells in vitro and in vivo. Methods The cytotoxicity of treatment on Walker-256 and SMMC-7721 tumor cells was determined using MIT assay in vitro with different dosages ofultrasonic disruption(42KHz, 0. 22W or 0.15W) plus carboplatin (0-100μg/ml), and the tumor growth was also observed in vivo after the Walker-256 cells, which had exposed to ultrasonic disruption with 42KHz, 0. 22Wand/or carboplatin of 60μg/ml, were injected into SD rats subcutaneously or injected into the portal vein duringoperation. Results Ultrasonic disruption enhanced markedly the cytotoxicity of carboplatin on these ho cell lines.The larger the dosage of the carboplatin and the higher the power output of the ultrasound were, the stronger wascytotoxicity. Tumor growth could not be induced in rats by subcutaneous injection or by injection into the liver withWalker-256 cells having exposed to ultrasound plus chemotherapy. Conclusions The therapy using ultrasonicdisruption plus chemotherapy may destroy the cancer cells completely so that it can prevent them from remote metastasis.
出处
《中华肝胆外科杂志》
CAS
CSCD
1999年第1期20-23,共4页
Chinese Journal of Hepatobiliary Surgery
基金
中国国家博士后基金
上海市医学发展基金
关键词
肝癌
门静脉癌栓
超声消融
药物疗法
Liver cancer Portal vein tumor emboli Ultrasonic disruption Chemotherapy
